The ADTRP gene encodes the Androgen-Dependent TFPI-Regulating Protein and is a susceptibility gene for contrary artery disease (CAD). We performed global gene expression profiling for ADTRP knockdown using microarrays in human HepG2 cells. Follow-up real-time RT-PCR analysis demonstrated that ADTRP regulates a diverse set of genes, including up-regulation of 7 histone genes, down-regulation of multiple cell cycle genes (CCND1, CDK4 and CDKN1A), and up-regulation of apoptosis genes (CASP7 and PDCD2) in HepG2 cells and endothelial cells. Consistently, ADTRP increases the number of S phase cells during cell cycle, promotes cell proliferation and inhibits apoptosis. Our study provides novel insights into the function of ADTRP and biological pathways involving ADTRP, which may be involved in the pathogenesis of CAD.
- global gene expression microarray
- coronary artery disease (CAD)
- cyclin D1
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