Physiol. Genomics Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 9: 5-13, 2002. First published February 12, 2002; doi:10.1152/physiolgenomics.00117.2001
1094-8341/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
9/1/5    most recent
00117.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Malley, S. E.
Right arrow Articles by Vizzard, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Malley, S. E.
Right arrow Articles by Vizzard, M. A.
Received 17 December 2001; accepted in final form 11 February 2002.
Physiological Genomics 9:5-13 (2002)
1094-8341/02 $5.00 © 2002 American Physiological Society

Changes in urinary bladder cytokine mRNA and protein after cyclophosphamide-induced cystitis

Susan E. Malley1 and Margaret A. Vizzard1,2

1 University of Vermont, College of Medicine, Departments of Neurology and Anatomy
2 Neurobiology, Burlington, Vermont 05405

Cyclophosphamide (CYP)-induced cystitis alters micturition function and produces reorganization of the micturition reflex. This reorganization may involve cytokine expression in the urinary bladder. These studies have determined candidate cytokines in the bladder that may contribute to the reorganization process. An RNase protection assay was used to measure changes in rat bladder cytokine mRNA [interferon-{gamma} (IFN)-{gamma}, interleukin-1{alpha}/ß (IL-1{alpha}/ß), IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, and tumor necrosis factor-{alpha}/ß (TNF-{alpha}/ß)] after acute (4 h), intermediate (48 h), or chronic (10 day) cystitis. The correlation between bladder cytokine mRNA and protein expression was also determined by immunoassay. Although at each time point after cystitis significant changes in bladder cytokine mRNA were observed, the magnitude differed (acute > intermediate > chronic). Acute cystitis demonstrated the most robust changes (P <= 0.005; IL-1ß, 330-fold increase; IL-2, 20-fold increase; IL-4, 8-fold increase; IL-6, 80-fold increase) in cytokine mRNA expression and TNF-{alpha} or TNF-ß mRNA were only increased (2–10-fold) after acute cystitis. More modest increases in cytokine mRNA expression were observed after 48-h or 10-day cystitis. Cytokine protein expression generally paralleled that of mRNA. Increased cytokine expression after CYP-induced cystitis, alone or in combination with other inflammatory mediators or growth factors, may contribute to altered lower urinary tract function after cystitis.

inflammation; interleukins; tumor necrosis factor; neuroimmune interactions




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
B. P. Cheppudira, B. M. Girard, S. E. Malley, K. C. Schutz, V. May, and M. A. Vizzard
Upregulation of vascular endothelial growth factor isoform VEGF-164 and receptors (VEGFR-2, Npn-1, and Npn-2) in rats with cyclophosphamide-induced cystitis
Am J Physiol Renal Physiol, September 1, 2008; 295(3): F826 - F836.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
P. L. Vera, X. Wang, and K. L. Meyer-Siegler
Upregulation of Macrophage Migration Inhibitory Factor (MIF) and CD74, Receptor for MIF, in Rat Bladder During Persistent Cyclophosphamide-Induced Inflammation
Experimental Biology and Medicine, May 1, 2008; 233(5): 620 - 626.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. B. Klinger, A. Dattilio, and M. A. Vizzard
Expression of cyclooxygenase-2 in urinary bladder in rats with cyclophosphamide-induced cystitis
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R677 - R685.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. A. Corrow and M. A. Vizzard
Phosphorylation of extracellular signal-regulated kinases in urinary bladder in rats with cyclophosphamide-induced cystitis
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R125 - R134.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L.-Y. Qiao and M. A. Gulick
Region-specific changes in the phosphorylation of ERK1/2 and ERK5 in rat micturition pathways following cyclophosphamide-induced cystitis
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1368 - R1375.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. LaBerge, S. E. Malley, K. Zvarova, and M. A. Vizzard
Expression of corticotropin-releasing factor and CRF receptors in micturition pathways after cyclophosphamide-induced cystitis
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R692 - R703.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
V. Y. Hu, S. Malley, A. Dattilio, J. B. Folsom, P. Zvara, and M. A. Vizzard
COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2003; 284(2): R574 - R585.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online