HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 33/1/65    most recent 00223.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Everts, R. E. Articles by Lewin, H. A. Search for Related Content PubMed PubMed Citation Articles by Everts, R. E. Articles by Lewin, H. A.

Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer

¹ Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois
² UMR Biologie du Développement et Reproduction, Institut National de la Recherche Agronomique, Jouy-en-Josas, France
³ Center for Regenerative Biology/Department of Animal Sciences, University of Connecticut, Storrs, Connecticut

Transcription profiling of placentomes derived from somatic cell nuclear transfer (SCNT, n = 20), in vitro fertilization (IVF, n = 9), and artificial insemination (AI, n = 9) at or near term development was performed to better understand why SCNT and IVF often result in placental defects, hydrops, and large offspring syndrome (LOS). Multivariate analysis of variance was used to distinguish the effects of SCNT, IVF, and AI on gene expression, taking into account the effects of parturition (term or preterm), sex of fetus, breed of dam, breed of fetus, and pathological finding in the offspring (hydrops, normal, or other abnormalities). Differential expression of 20 physiologically important genes was confirmed with quantitative PCR. The largest effect on placentome gene expression was attributable to whether placentas were collected at term or preterm (i.e., whether the collection was because of disease or to obtain stage-matched controls) followed by placentome source (AI, IVF, or SCNT). Gene expression in SCNT placentomes was dramatically different from AI (n = 336 genes; 276 >2-fold) and from IVF (n = 733 genes; 162 >2-fold) placentomes. Functional analysis of differentially expressed genes (DEG) showed that IVF has significant effects on genes associated with cellular metabolism. In contrast, DEG associated with SCNT are involved in multiple pathways, including cell cycle, cell death, and gene expression. Many DEG were shared between the gene lists for IVF and SCNT comparisons, suggesting that common pathways are affected by the embryo culture methods used for IVF and SCNT. However, the many unique gene functions and pathways affected by SCNT suggest that cloned fetuses may be starved and accumulating toxic wastes due to placental insufficiency caused by reprogramming errors. Many of these genes are candidates for hydrops and LOS.

placenta; bovine; reprogramming

This article has been cited by other articles:

				A. Eberlein, A. Takasuga, K. Setoguchi, R. Pfuhl, K. Flisikowski, R. Fries, N. Klopp, R. Furbass, R. Weikard, and C. Kuhn Dissection of Genetic Factors Modulating Fetal Growth in Cattle Indicates a Substantial Role of the Non-SMC Condensin I Complex, Subunit G (NCAPG) Gene Genetics, November 1, 2009; 183(3): 951 - 964. [Abstract] [Full Text] [PDF]

				N. Mansouri-Attia, J. Aubert, P. Reinaud, C. Giraud-Delville, G. Taghouti, L. Galio, R. E. Everts, S. Degrelle, C. Richard, I. Hue, et al. Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation Physiol Genomics, September 1, 2009; 39(1): 14 - 27. [Abstract] [Full Text] [PDF]

				N. Mansouri-Attia, O. Sandra, J. Aubert, S. Degrelle, R. E. Everts, C. Giraud-Delville, Y. Heyman, L. Galio, I. Hue, X. Yang, et al. Endometrium as an early sensor of in vitro embryo manipulation technologies PNAS, April 7, 2009; 106(14): 5687 - 5692. [Abstract] [Full Text] [PDF]

				P. Murphey, Y. Yamazaki, C. A. McMahan, C. A. Walter, R. Yanagimachi, and J. R. McCarrey Epigenetic regulation of genetic integrity is reprogrammed during cloning PNAS, March 24, 2009; 106(12): 4731 - 4735. [Abstract] [Full Text] [PDF]