HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 30/2/95    most recent 00211.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Mashimo, T. Articles by Nabika, T. Search for Related Content PubMed PubMed Citation Articles by Mashimo, T. Articles by Nabika, T.

Comprehensive QTL analysis of serum cholesterol levels before and after a high-cholesterol diet in SHRSP

¹ Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan
² Department of Hygiene, Kinki University School of Medicine, Osakasayama, Japan
³ Department of Functional Pathology, Shimane University School of Medicine, Shimane University, Izumo, Japan
⁴ Department of Laboratory Medicine, Shimane University School of Medicine, Shimane University, Izumo, Japan
⁵ Department of Experimental Animals, Center for Integrated Research in Science, Shimane University, Izumo, Japan
⁶ International Center for Research on Primary Prevention of Cardiovascular Diseases, Kyoto, Japan

The stroke-prone spontaneously hypertensive rat (SHRSP) showed an exaggerated response to a high-fat, high-cholesterol (HFC) diet, and the resulting reactive hypercholesterolemia was suggested to exacerbate the atherogenic process in this rat. We thus performed a quantitative trait locus (QTL) analysis on the serum cholesterol level of SHRSP before and after the HFC diet, with the final goal being the identification of the genetic mechanisms of its reactive hypercholesterolemia. Three hundred fifty-eight F2 rats between SHRSP and Wistar-Kyoto rat were employed in the study. The serum cholesterol and apoprotein E were measured before and after 2 wk of feeding with the HFC diet. Multiple QTLs for the basal cholesterol level were identified on chromosomes 1 and 5, whereas those for the postdietary cholesterol level were on chromosomes 7, 15, and 16. The cholesterol QTLs before and after HFC diet did not overlap with one another, implying that the involved metabolic processes were considerably different between the two conditions. Supporting this, VLDL and LDL cholesterol were the major components of the postdietary serum cholesterol, whereas the basal cholesterol level consisted mainly of HDL cholesterol. A substantial difference of the QTLs between males and females was observed, especially after the HFC diet. The QTL on chromosome 15 had an inverse effect on the cholesterol level, suggesting that the congenic substitution of the SHRSP fragment with that of Wistar-Kyoto rats could induce a greater cholesterol level in SHRSP. This observation is significant in establishing a new model for atherosclerosis with hypertension in rats.

genetics; hypercholesterolemia; quantitative trait locus; apoprotein E; stroke-prone spontaneously hypertensive rat