Physiol. Genomics Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 3: 93-99, 2000;
1094-8341/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (111)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by STEARS, R. L.
Right arrow Articles by GULLANS, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by STEARS, R. L.
Right arrow Articles by GULLANS, S. R.
Received 3 May 2000; accepted in final form 5 June 2000.
Physiological Genomics 3:93-99 (2000)
1094-8341/00 $5.00 © 2000 American Physiological Society

A novel, sensitive detection system for high-density microarrays using dendrimer technology

ROBIN L. STEARS1, ROBERT C. GETTS2 and STEVEN R. GULLANS1

1 Brigham and Women’s Hospital, Department of Medicine, Harvard Institutes of Medicine, Boston, Massachusetts 02115
2 Genisphere, Philadelphia, Pennsylvania 19131

Stears, Robin L., Robert C. Getts, and Steven R. Gullans. A novel, sensitive detection system for high-density microarrays using dendrimer technology. Physiol Genomics 3: 93–99, 2000.—To improve signal detection on cDNA microarrays, we adapted a fluorescent oligonucleotide dendrimeric signal amplification system to microarray technology. This signal detection method requires 16-fold less RNA for probe synthesis, does not depend on the incorporation of fluorescent dNTPs into a reverse transcription reaction, generates a high signal-to-background ratio, and can be used to allow for multichannel detection on a single chip. Furthermore, since the dendrimers can be detected individually, it may be possible, by employing dendrimer-binding standards, to calculate the numbers of bound cDNAs can be estimated. These features make the dendrimer signal detection reagent ideal for high-throughput functional genomics research.

high-density DNA microarrays; dendrimers; signal detection




This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
C. R. Sabanayagam and J. R. Lakowicz
Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles
Nucleic Acids Res., January 28, 2007; 35(2): e13 - e13.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Nygaard and E. Hovig
Options available for profiling small samples: a review of sample amplification technology when combined with microarray profiling
Nucleic Acids Res., February 9, 2006; 34(3): 996 - 1014.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
M. Bredel, C. Bredel, D. Juric, G. E. Duran, R. X. Yu, G. R. Harsh, H. Vogel, L. D. Recht, A. C. Scheck, and B. I. Sikic
Tumor Necrosis Factor-{alpha}-Induced Protein 3 As a Putative Regulator of Nuclear Factor-{kappa}B-Mediated Resistance to O6-Alkylating Agents in Human Glioblastomas
J. Clin. Oncol., January 10, 2006; 24(2): 274 - 287.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Bredel, C. Bredel, D. Juric, G. R. Harsh, H. Vogel, L. D. Recht, and B. I. Sikic
Functional Network Analysis Reveals Extended Gliomagenesis Pathway Maps and Three Novel MYC-Interacting Genes in Human Gliomas
Cancer Res., October 1, 2005; 65(19): 8679 - 8689.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. K. Borucki, J. Reynolds, D. R. Call, T. J. Ward, B. Page, and J. Kadushin
Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA
J. Clin. Microbiol., July 1, 2005; 43(7): 3255 - 3259.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Singh, R. J. Maganti, S. V. Jabba, M. Wang, G. Deng, J. D. Heath, N. Kurn, and P. Wangemann
Microarray-based comparison of three amplification methods for nanogram amounts of total RNA
Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1179 - C1189.
[Abstract] [Full Text] [PDF]

Home page
 aacredbookHome page
J. W. Jacobson
Comprehensive Molecular Analysis of Human Specimens
Am. Assoc. Cancer Res. Educ. Book, April 1, 2005; 2005(1): 311 - 314.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Liu, C. Liang, Z. Liang, K. Tus, and E. K. Wakeland
Sle1ab Mediates the Aberrant Activation of STAT3 and Ras-ERK Signaling Pathways in B Lymphocytes
J. Immunol., February 1, 2005; 174(3): 1630 - 1637.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. L. Afonso, M. E. Piccone, K. M. Zaffuto, J. Neilan, G. F. Kutish, Z. Lu, C. A. Balinsky, T. R. Gibb, T. J. Bean, L. Zsak, et al.
African Swine Fever Virus Multigene Family 360 and 530 Genes Affect Host Interferon Response
J. Virol., February 15, 2004; 78(4): 1858 - 1864.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Liang, A. W. Cowley Jr, and A. S. Greene
High throughput gene expression profiling: a molecular approach to integrative physiology
J. Physiol., January 1, 2004; 554(1): 22 - 30.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Romualdi, S. Trevisan, B. Celegato, G. Costa, and G. Lanfranchi
Improved detection of differentially expressed genes in microarray experiments through multiple scanning and image integration
Nucleic Acids Res., December 1, 2003; 31(23): e149 - e149.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Petalidis, S. Bhattacharyya, G. A. Morris, V. P. Collins, T. C. Freeman, and P. A. Lyons
Global amplification of mRNA by template-switching PCR: linearity and application to microarray analysis
Nucleic Acids Res., November 15, 2003; 31(22): e142 - e142.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
F. Yadetie, A. Laegreid, I. Bakke, W. Kusnierczyk, J. Komorowski, H. L. Waldum, and A. K. Sandvik
Liver gene expression in rats in response to the peroxisome proliferator-activated receptor-{alpha} agonist ciprofibrate
Physiol Genomics, September 29, 2003; 15(1): 9 - 19.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
K. Dobbin, J. H. Shih, and R. Simon
Questions and Answers on Design of Dual-Label Microarrays for Identifying Differentially Expressed Genes
J Natl Cancer Inst, September 17, 2003; 95(18): 1362 - 1369.
[Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. C. Xiang, M. Chen, L. Ma, Q. N. Phan, J. M. Inman, O. A. Kozhich, and M. J. Brownstein
A new strategy to amplify degraded RNA from small tissue samples for microarray studies
Nucleic Acids Res., May 1, 2003; 31(9): e53 - e53.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Gupta, A. Cherkassky, P. Chatis, R. Joseph, A. L. Johnson, J. Broadbent, T. Erickson, and J. DiMeo
Directly labeled mRNA produces highly precise and unbiased differential gene expression data
Nucleic Acids Res., February 15, 2003; 31(4): e13 - e13.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Smith, P. Underhill, C. Pritchard, Z. Tymowska-Lalanne, S. Abdul-Hussein, H. Hilton, L. Winchester, D. Williams, T. Freeman, S. Webb, et al.
Single primer amplification (SPA) of cDNA for microarray expression analysis
Nucleic Acids Res., February 1, 2003; 31(3): e9 - e9.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Sanchez-Carbayo, P. Capodieci, and C. Cordon-Cardo
Tumor Suppressor Role of KiSS-1 in Bladder Cancer: Loss of KiSS-1 Expression Is Associated with Bladder Cancer Progression and Clinical Outcome
Am. J. Pathol., February 1, 2003; 162(2): 609 - 617.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Sanchez-Carbayo
Use of High-Throughput DNA Microarrays to Identify Biomarkers for Bladder Cancer
Clin. Chem., January 1, 2003; 49(1): 23 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Sanchez-Carbayo, N. D. Socci, E. Charytonowicz, M. Lu, M. Prystowsky, G. Childs, and C. Cordon-Cardo
Molecular Profiling of Bladder Cancer Using cDNA Microarrays: Defining Histogenesis and Biological Phenotypes
Cancer Res., December 1, 2002; 62(23): 6973 - 6980.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
E. Manduchi, L. M. Scearce, J. E. Brestelli, G. R. Grant, K. H. Kaestner, and C. J. Stoeckert JR.
Comparison of different labeling methods for two-channel high-density microarray experiments
Physiol Genomics, September 3, 2002; 10(3): 169 - 179.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. M. Scearce, J. E. Brestelli, S. K. McWeeney, C. S. Lee, J. Mazzarelli, D. F. Pinney, A. Pizarro, C. J. Stoeckert Jr., S. W. Clifton, M. A. Permutt, et al.
Functional Genomics of the Endocrine Pancreas: The Pancreas Clone Set and PancChip, New Resources for Diabetes Research
Diabetes, July 1, 2002; 51(7): 1997 - 2004.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
D. R. Dorris, R. Ramakrishnan, D. Trakas, F. Dudzik, R. Belval, C. Zhao, A. Nguyen, M. Domanus, and A. Mazumder
A Highly Reproducible, Linear, and Automated Sample Preparation Method for DNA Microarrays
Genome Res., June 1, 2002; 12(6): 976 - 984.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. K. Chauhan, N. A. Reed, W. Zhang, M. K. Duncan, M. W. Kilimann, and A. Cvekl
Identification of Genes Downstream of Pax6 in the Mouse Lens Using cDNA Microarrays
J. Biol. Chem., March 22, 2002; 277(13): 11539 - 11548.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Ivashuta, K. Uchiyama, M. Gau, and Y. Shimamoto
Linear amplification coupled with controlled extension as a means of probe amplification in a cDNA array and gene expression analysis during cold acclimation in alfalfa (Medicago sativa L.)
J. Exp. Bot., February 1, 2002; 53(367): 351 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Nallur, C. Luo, L. Fang, S. Cooley, V. Dave, J. Lambert, K. Kukanskis, S. Kingsmore, R. Lasken, and B. Schweitzer
Signal amplification by rolling circle amplification on DNA microarrays
Nucleic Acids Res., December 1, 2001; 29(23): e118 - e118.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. KURELLA, L.-L. HSIAO, T. YOSHIDA, J. D. RANDALL, G. CHOW, S. S. SARANG, R. V. JENSEN, and S. R. GULLANS
DNA Microarray Analysis of Complex Biologic Processes
J. Am. Soc. Nephrol., May 1, 2001; 12(5): 1072 - 1078.
[Abstract] [Full Text]

Home page
 DiabetesHome page
L. M. Scearce, J. E. Brestelli, S. K. McWeeney, C. S. Lee, J. Mazzarelli, D. F. Pinney, A. Pizarro, C. J. Stoeckert Jr., S. W. Clifton, M. A. Permutt, et al.
Functional Genomics of the Endocrine Pancreas: The Pancreas Clone Set and PancChip, New Resources for Diabetes Research
Diabetes, July 1, 2002; 51(7): 1997 - 2004.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online