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Physiol. Genomics 29: 253-259, 2007. First published January 30, 2007; doi:10.1152/physiolgenomics.00067.2006 Free Article
1094-8341/07 $8.00
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Received 18 April 2006; accepted in final form 23 January 2007.
Physiological Genomics 29:253-259 (2007)
1094-8341/07 $8.00 © 2007 American Physiological Society

MPSS profiling of embryonic gonad and primordial germ cells in chicken

Heebal Kim1,4, Tae Sub Park2, Woon Kyu Lee3, Sunjin Moon1, Jin Nam Kim2, Ji Hye Shin2, Jin Gyoung Jung1,2, Seon Duk Lee2, Sang Hyun Park1, Kyung Je Park1, Mi A Kim2, Sang Su Shin1, Tae Min Kim1, Jungrye Nam1, Yeonkyung Kang2, Jeong Mook Lim1 and Jae Yong Han1

1 Department of Agricultural Biotechnology, Seoul National University, Seoul
2 Department of Genomics, Avicore Biotechnology Institute, Gyeonggi-do
3 Department of Laboratory Animal, Medical Research Center, College of Medicine, Yonsei University
4 Graduate Program in Bioinformatics, Seoul National University, Seoul, Korea

The massively parallel signature sequencing (MPSS) provides a greater depth of coverage than expressed sequence tag scan or microarray and provides a comprehensive expression profile. We used the MPSS technology to uncover gene expression profiling in the early embryonic gonads and primordial germ cells (PGCs) in the chicken. Total numbers of sequenced signatures were 1,012,533 and 995,676 for the PGCs and gonad, respectively. Using a noise distribution model, we found that 1.67% of all signatures are expressed at a higher level in PCGs and 2.81% of all signatures are expressed at a higher level in the gonad. The MPSS data are presented via an interactive web interface available at http://snugenome.snu.ac.kr/MPSS. The MPSS data have been submitted to the Gene Expression Omnibus of the National Center for Biotechnology Information (accession number GSM137300 and GSM137301 for PGCs and gonad, respectively).

massively parallel signature sequencing






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