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Insights into the immune transcriptome of the shrimp Litopenaeus vannamei: tissue-specific expression profiles and transcriptomic responses to immune challenge

¹ Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, Hollings Marine Laboratory, Charleston, South Carolina
² Department of Biostatistics and Applied Mathematics, MD Anderson Cancer Center, Houston, Texas
³ Department of Biostatistics, Bioinformatics and Epidemiology, Medical University of South Carolina
⁴ Marine Resources Research Institute, South Carolina Department of Natural Resources
⁵ Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina

Infectious disease constitutes a major obstacle to the sustainability of shrimp aquaculture worldwide and a significant threat to natural populations of shrimp and other crustacea. The study of the shrimp immune system, including the response to viral infection, has been hampered by a relative lack of molecular genetic information and of tools suitable for high-throughput assessment of gene expression. In this report, the generation of a cDNA microarray encompassing 2,469 putative unigenes expressed in gills, circulating hemocytes, and hepatopancreas of Litopenaeus vannamei is described. The unigenes printed on the microarray were derived from the analyses of 7,021 expressed sequence tags obtained from standard cDNA libraries as well as from libraries generated by suppression subtractive hybridization, after challenging shrimp with a variety of immune stimuli. The general utility of the cDNA microarray was demonstrated by interrogating the array with labeled RNA from four different shrimp tissues (gills, hemocytes, hepatopancreas, and muscle) and by analyzing the transcriptomic response of shrimp to a lethal challenge with white spot syndrome virus. Our results indicate that white spot syndrome virus infection upregulates (in the hepatopancreas) genes encoding known and potential antimicrobial effectors, while some genes involved in protection from oxidative stress were found to be downregulated by the virus.

cDNA microarray; immune response; subtractive hybridization; expressed sequence tags; white spot syndrome virus