HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Physiol. Genomics 25: 414-425, 2006. First published February 21, 2006; doi:10.1152/physiolgenomics.00248.2005
1094-8341/06 $8.00

This Article Free upon publication Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 25/3/414    most recent 00248.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Ellestad, L. E. Articles by Porter, T. E. Search for Related Content PubMed PubMed Citation Articles by Ellestad, L. E. Articles by Porter, T. E.

Gene expression profiling during cellular differentiation in the embryonic pituitary gland using cDNA microarrays

¹ Department of Animal and Avian Sciences University of Maryland, College Park, Maryland
² Molecular and Cell Biology Program, University of Maryland, College Park, Maryland
³ Department of Animal and Food Sciences, University of Delaware, Newark, Delaware

The anterior pituitary is comprised of five major hormone-secreting cell types that differentiate during embryonic development in a temporally distinct manner. Microarrays containing 5,128 unique cDNAs expressed in the chicken neuroendocrine system were produced and used to identify genes with potential involvement in the onset of thyroid-stimulating hormone ß-subunit (TSHß), growth hormone (GH), and prolactin (PRL) mRNA during embryonic development. We identified 352 cDNAs that were differentially expressed (P 0.05) on embryonic day 10 (e10), e12, e14, or e17, the period of thyrotroph, somatotroph, and lactotroph differentiation. Self-organizing maps were used to identify genes that may function to initiate hormone gene transcription. Consistent with cellular ontogeny, TSHß mRNA increased steadily between e10 and e17, GH mRNA increased between e12 and e17, and PRL mRNA did not increase until e17. Expression of 141 genes increased in a manner similar to TSHß mRNA, and 64 genes decreased between e10 and e17. Although genes with these expression profiles are likely involved in development of the pituitary gland as a whole, some of these could be specifically associated with thyrotroph differentiation. Similarly, the expression profiles of 69 and 61 genes indicate a potential involvement in the induction of GH and PRL mRNA, respectively. Quantitative real-time RT-PCR was used to confirm microarray results for 31 genes. This is the first study to evaluate changes in anterior pituitary gene expression during embryonic development of any species using microarrays, and numerous transcription factors and signaling molecules not previously implicated in pituitary development were identified.

embryo; thyrotropin; somatotropin; prolactin

This article has been cited by other articles:

				L. E Ellestad, S. A Malkiewicz, H D. Guthrie, G. R Welch, and T. E Porter Expression and regulation of glucocorticoid-induced leucine zipper in the developing anterior pituitary gland J. Mol. Endocrinol., February 1, 2009; 42(2): 171 - 183. [Abstract] [Full Text] [PDF]

				F. Z. Lu, X. X. Wang, Q. X. Pan, R. H. Huang, and H. L. Liu Expression of Genes Involved in the Somatotropic, Thyrotropic, and Corticotropic Axes During Development of Langshan and Arbor Acres Chickens Poult. Sci., October 1, 2008; 87(10): 2087 - 2097. [Abstract] [Full Text] [PDF]

				L. A. Cogburn, T. E. Porter, M. J. Duclos, J. Simon, S. C. Burgess, J. J. Zhu, H. H. Cheng, J. B. Dodgson, and J. Burnside Functional Genomics of the Chicken A Model Organism Poult. Sci., October 1, 2007; 86(10): 2059 - 2094. [Abstract] [Full Text] [PDF]

				S. A. Jenkins, M. Muchow, M. P. Richards, J. P. McMurtry, and T. E. Porter Administration of Adrenocorticotropic Hormone during Chicken Embryonic Development Prematurely Induces Pituitary Growth Hormone Cells Endocrinology, August 1, 2007; 148(8): 3914 - 3921. [Abstract] [Full Text] [PDF]

				J. B. Dodgson The Chicken Genome: Some Good News and Some Bad News Poult. Sci., July 1, 2007; 86(7): 1453 - 1459. [Abstract] [Full Text] [PDF]

				D. W. Burt Emergence of the Chicken as a Model Organism: Implications for Agriculture and Biology Poult. Sci., July 1, 2007; 86(7): 1460 - 1471. [Abstract] [Full Text] [PDF]

				C. F. Yen, H. W. Lin, J. C. Hsu, C. Lin, T. F. Shen, and S. T. Ding The Expression of Pituitary Gland Genes in Laying Geese Poult. Sci., December 1, 2006; 85(12): 2265 - 2269. [Abstract] [Full Text] [PDF]