HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Physiol. Genomics 25: 250-262, 2006. First published January 17, 2006; doi:10.1152/physiolgenomics.00231.2005
1094-8341/06 $8.00

This Article Free upon publication Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 25/2/250    most recent 00231.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Costa, M. Articles by Coceani, F. Search for Related Content PubMed PubMed Citation Articles by Costa, M. Articles by Coceani, F.

Gene expression in ductus arteriosus and aorta: comparison of birth and oxygen effects

¹ Institute of Neuroscience, Consiglio Nazionale delle Ricerche (CNR), Pisa, Italy
² Scuola Superiore S. Anna, CNR, Pisa, Italy
³ Institute of Clinical Physiology, CNR, Pisa, Italy
⁴ Computational Biology Center, Memorial Sloan-Kettering Cancer Center, New York, New York
⁵ Dulbecco Telethon Institute and Department of Endocrinology, University of Pisa, Pisa, Italy

Ductus arteriosus (DA) closure is initiated by oxygen rise postnatally and progresses in two, functional-to-permanent, stages. Here, using GeneChip Arrays in rats (normoxic and hyperoxic fetus, normoxic newborn), we examined whether oxygen alone duplicates the birth process in affecting DA genes. In addition, by comparing DA with aorta (Ao), we identified features in postnatal gene profile marking transitional adjustments in a closing (DA) vs. a persistent (Ao) vessel. We found changes in neonatal DA denoting enhanced formation and action of the constrictor endothelin-1 (ET-1). Likewise, ANG II type 1 receptor was upregulated, and the compound was a constrictor. Conversely, relaxant PGE₂ became less effective. Among agents for functional closure, only ET-1 was affected similarly by oxygen and birth. Coincidentally, neonatal DA showed enhanced contractile drive with upregulation of Rho-Rho kinase and calcium signaling along with downregulation of contractile proteins. The latter effect was shared by oxygen. Changes denoting active remodeling were also seen in neonatal but not hyperoxic fetal DA. Ao, unlike DA, exhibited postnatal variations in noradrenergic, purinergic, and PGI₂ systems with opposing effects on vasomotion. Contraction and remodeling processes were also less affected by birth, whereas lipid and glucose metabolism were upregulated. We conclude that several agents, including ANG II as novel effector, promote functional closure of DA, but only ET-1 is causally coupled with oxygen. Oxygen has no role in processes for permanent closure. Functional closure is associated with downregulation of contractile apparatus, and this may render neonatal DA less amenable to tone manipulation. Conceivably, activation of metabolism in neonatal Ao is a distinguishing feature for transitional adaptations in the permanent vasculature.

ductus closure; postnatal programming; fetal and neonatal physiology

This article has been cited by other articles:

				B. Baragatti, M. L. Schwartzman, D. Angeloni, F. Scebba, E. Ciofini, D. Sodini, V. Ottaviano, S. Nencioni, A. Paolicchi, J. P. Graves, et al. EDHF function in the ductus arteriosus: evidence against involvement of epoxyeicosatrienoic acids and 12S-hydroxyeicosatetraenoic acid Am J Physiol Heart Circ Physiol, December 1, 2009; 297(6): H2161 - H2168. [Abstract] [Full Text] [PDF]

				R. I. Clyman, N. Waleh, H. Kajino, C. Roman, and F. Mauray Calcium-dependent and calcium-sensitizing pathways in the mature and immature ductus arteriosus Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1650 - R1656. [Abstract] [Full Text] [PDF]

				U. Yokoyama, Y. Sato, T. Akaike, S. Ishida, J. Sawada, T. Nagao, H. Quan, M. Jin, M. Iwamoto, S. Yokota, et al. Maternal vitamin A alters gene profiles and structural maturation of the rat ductus arteriosus Physiol Genomics, September 11, 2007; 31(1): 139 - 157. [Abstract] [Full Text] [PDF]

				H. Kajimoto, K. Hashimoto, S. N. Bonnet, A. Haromy, G. Harry, R. Moudgil, T. Nakanishi, I. Rebeyka, B. Thebaud, E. D. Michelakis, et al. Oxygen Activates the Rho/Rho-Kinase Pathway and Induces RhoB and ROCK-1 Expression in Human and Rabbit Ductus Arteriosus by Increasing Mitochondria-Derived Reactive Oxygen Species: A Newly Recognized Mechanism for Sustaining Ductal Constriction Circulation, April 3, 2007; 115(13): 1777 - 1788. [Abstract] [Full Text] [PDF]

				Z. Hong, F. Hong, A. Olschewski, J. A. Cabrera, A. Varghese, D. P. Nelson, and E. K. Weir Role of Store-Operated Calcium Channels and Calcium Sensitization in Normoxic Contraction of the Ductus Arteriosus Circulation, September 26, 2006; 114(13): 1372 - 1379. [Abstract] [Full Text] [PDF]