HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 25/1/75    most recent 00188.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by van Erp, K. Articles by Hoffmann, R. Search for Related Content PubMed PubMed Citation Articles by van Erp, K. Articles by Hoffmann, R.

Role of strain differences on host resistance and the transcriptional response of macrophages to infection with Yersinia enterocolitica

Max von Pettenkofer-Institut, Department of Bacteriology, Munich, Germany

The outcome of a host-pathogen encounter is determined by virulence factors of the pathogen and defense factors of the host. We characterized the impact of host factors [resistant (C57BL/6) or susceptible (BALB/c) genetic background and exposure to interferon (IFN)-] on transcriptional responses of bone marrow-derived macrophages (BMDM) to infection with Yersinia enterocolitica. IFN- treatment more profoundly altered the transcriptome of BMDM than did bacterial infection or genetic background. In BALB/c BMDM, 1,161 genes were differentially expressed in response to Yersinia infection with or without IFN- prestimulation. Fourteen genes (1.2%) could only be induced by BALB/c BMDM in response to Yersinia infection after IFN- pretreatment. These genes inhibit apoptosis, activate NF-B and Erk signaling, are chemotactic to neutrophils, and are involved in cytoskeletal reorganization, hence possibly in phagocytosis. Ten of these genes possess a common module of binding sites for Hox, Pou, and Creb transcription factors in 2 kb of upstream genomic sequence, suggesting a possible novel role of these transcription factors in regulation of immune responses. Fifty-two of one thousand fifty differentially expressed genes (4.9%) were induced more strongly by C57BL/6 BMDM in response to Yersinia infection than BALB/c BMDM. These genes activate NK cells, have antibacterial properties, or are involved in sensing chemokines and lipopolysaccharide (LPS). These data show that host resistance factors modulate a surprisingly small, but identifiable and functionally significant, portion of the macrophage transcriptome in response to Yersinia infection.

bacterial infection; host response; BALB/c; C57BL/6

This article has been cited by other articles:

				M. Benoit, B. Desnues, and J.-L. Mege Macrophage Polarization in Bacterial Infections J. Immunol., September 15, 2008; 181(6): 3733 - 3739. [Abstract] [Full Text] [PDF]

				L. R. Hoffman and S. I. Miller The systems biology of infection in animal models bears fruit PNAS, June 20, 2006; 103(25): 9377 - 9378. [Full Text] [PDF]