HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 23/2/182    most recent 00139.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Sarropoulou, E. Articles by Geisler, R. Search for Related Content PubMed PubMed Citation Articles by Sarropoulou, E. Articles by Geisler, R.

Article

Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology

¹ Hellenic Center for Marine Research, Institute for Marine Biology and Genetics, Iraklio, Crete, Greece; ² Center for Marine Science, Universidade do Algarve, Faro, Portugal; and ³ Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Germany

ABSTRACT

Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices.

Sparus auratus; stress response

This article has been cited by other articles:

				R. Franch, B. Louro, M. Tsalavouta, D. Chatziplis, C. S. Tsigenopoulos, E. Sarropoulou, J. Antonello, A. Magoulas, C. C. Mylonas, M. Babbucci, et al. A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L. Genetics, October 1, 2006; 174(2): 851 - 861. [Abstract] [Full Text] [PDF]

				R. N. Morrison, G. A. Cooper, B. F. Koop, M. L. Rise, A. R. Bridle, M. B. Adams, and B. F. Nowak Transcriptome profiling the gills of amoebic gill disease (AGD)-affected Atlantic salmon (Salmo salar L.): a role for tumor suppressor p53 in AGD pathogenesis? Physiol Genomics, September 14, 2006; 26(1): 15 - 34. [Abstract] [Full Text] [PDF]