HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Figure All Versions of this Article: 22/2/257    most recent 00063.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Chen, S. H. Articles by Dumont, D. J. Search for Related Content PubMed PubMed Citation Articles by Chen, S. H. Articles by Dumont, D. J.

Gene expression analysis of Tek/Tie2 signaling

¹ Division of Molecular and Cellular Biology Research, Sunnybrook and Women's Research Institute, Toronto
² Department of Medical Biophysics, University of Toronto, Toronto
³ Toronto-Sunnybrook Regional Cancer Centre, Toronto
⁴ Heart and Stroke/Richard Lewar Centre of Excellence, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

The elaboration of the vasculature during embryonic development involves restructuring of the early vessels into a more complex vascular network. Of particular importance to this vascular remodeling process is the requirement of the Tek/Tie2 receptor tyrosine kinase. Mouse gene-targeting studies have shown that the Tie2-deficient embryos succumb to embryonic death at midgestation due to insufficient sprouting and remodeling of the primary capillary plexus. To identify the underlying genetic mechanisms regulating the process of vascular remodeling, transcriptomes modulated by Tie2 signaling were analyzed utilizing serial analysis of gene expression (SAGE). Two libraries were constructed and sequenced using embryonic day 8.5 yolk sac tissues from Tie2 wild-type and the Tie2-null littermates. After tag extraction, 45,689 and 45,275 SAGE tags were obtained for the Tie2 wild-type and Tie2-null libraries, respectively, yielding a total of 21,376 distinct tags. Close to 62% of the tags were uniquely annotated, whereas 10% of the total tags were unknown. Using semiquantitative PCR, the differential expression of eight genes was confirmed that included Elk3, an important angiogenic switch gene which was upregulated in the absence of Tie2 signaling. The results of this study provide valuable insight into the potential association between Tie2 signaling and other known angiogenic pathways as well as genes that might have novel functions in vascular remodeling.

receptor tyrosine kinase; angiogenesis; serial analysis of gene expression

This article has been cited by other articles:

				X.-H. Ma, S.-J. Hu, H. Ni, Y.-C. Zhao, Z. Tian, J.-L. Liu, G. Ren, X.-H. Liang, H. Yu, P. Wan, et al. Serial Analysis of Gene Expression in Mouse Uterus at the Implantation Site J. Biol. Chem., April 7, 2006; 281(14): 9351 - 9360. [Abstract] [Full Text] [PDF]

				M. Liang and B. Ventura Physiological genomics in PG and beyond: July to September 2005 Physiol Genomics, October 17, 2005; 23(2): 119 - 124. [Full Text] [PDF]