HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Physiol. Genomics 20: 244-255, 2005. First published December 14, 2004; doi:10.1152/physiolgenomics.00135.2004
1094-8341/05 $8.00

This Article Free upon publication Full Text Full Text (PDF) Supplemental Table All Versions of this Article: 20/3/244    most recent 00135.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Moreilhon, C. Articles by Barbry, P. Search for Related Content PubMed PubMed Citation Articles by Moreilhon, C. Articles by Barbry, P.

Live Staphylococcus aureus and bacterial soluble factors induce different transcriptional responses in human airway cells

¹ Institut de Pharmacologie Moléculaire et Cellulaire UMR 6097 Centre National de la Recherche Scientifique, Université de Nice-Sophia Antipolis, Valbonne
² Institut National de la Santé et de la Recherche Médicale (INSERM) UMR-S 514, IFR 53, CHU Hôpital Maison Blanche, Reims
³ INSERM U576, Hôpital de l’Archet, Nice
⁴ INSERM EMI 10014, Vandoeuvre-les-Nancy, France

To characterize the response of respiratory epithelium to infection by Staphylococcus aureus (S. aureus), human airway cells were incubated for 1 to 24 h with a supernatant of a S. aureus culture (bacterial supernatant), then profiled with a pangenomic DNA microarray. Because an upregulation of many genes was noticed around 3 h, three independent approaches were then used to characterize the host response to a 3-h contact either with bacterial supernatant or with live bacteria: 1) a DNA microarray containing 4,200 sequence-verified probes, 2) a semiquantitative RT-PCR with a set of 537 pairs of validated primers, or 3) ELISA assay of IL-8, IL-6, TNF, and PGE₂. Among others, Fos, Jun, and EGR-1 were upregulated by the bacterial supernatant and by live bacteria. Increased expression of bhlhb2 and Mig-6, promoter regions which harbor HIF responding elements, was explained by an increased expression of the HIF-1 protein. Activation of the inducible form of cyclooxygenase, COX-2, and of the interleukins IL-1, IL-6, and IL-8, as well as of the NF-B pathway, was observed preferentially in cells in contact with bacterial supernatant. Early infection was characterized by an upregulation of anti-apoptotic genes and a downregulation of pro-apoptotic genes. This correlated with a necrotic, rather than apoptotic cell death. Overall, this first global description of an airway epithelial infection by S. aureus demonstrates a larger global response to bacterial supernatant (in term of altered genes and variation factors) than to exponentially growing live bacteria.

transcriptome; microarray; inflammation; infection

This article has been cited by other articles:

				K. Dach, J. Zovko, M. Hogardt, I. Koch, K. van Erp, J. Heesemann, and R. Hoffmann Bacterial Toxins Induce Sustained mRNA Expression of the Silencing Transcription Factor klf2 via Inactivation of RhoA and Rhophilin 1 Infect. Immun., December 1, 2009; 77(12): 5583 - 5592. [Abstract] [Full Text] [PDF]

				S. Grosso, A. Puissant, M. Dufies, P. Colosetti, A. Jacquel, K. Lebrigand, P. Barbry, M. Deckert, J. P. Cassuto, B. Mari, et al. Gene expression profiling of imatinib and PD166326-resistant CML cell lines identifies Fyn as a gene associated with resistance to BCR-ABL inhibitors Mol. Cancer Ther., July 1, 2009; 8(7): 1924 - 1933. [Abstract] [Full Text] [PDF]

				G. Fitsialos, A.-A. Chassot, L. Turchi, M. A. Dayem, K. LeBrigand, C. Moreilhon, G. Meneguzzi, R. Busca, B. Mari, P. Barbry, et al. Transcriptional Signature of Epidermal Keratinocytes Subjected to in Vitro Scratch Wounding Reveals Selective Roles for ERK1/2, p38, and Phosphatidylinositol 3-Kinase Signaling Pathways J. Biol. Chem., May 18, 2007; 282(20): 15090 - 15102. [Abstract] [Full Text] [PDF]

				J. B. Vos, N. A. Datson, K. F. Rabe, and P. S. Hiemstra Exploring host-pathogen interactions at the epithelial surface: application of transcriptomics in lung biology Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L367 - L377. [Abstract] [Full Text] [PDF]

				E. P. O'Grady, H. Mulcahy, J. O'Callaghan, C. Adams, and F. O'Gara Pseudomonas aeruginosa Infection of Airway Epithelial Cells Modulates Expression of Kruppel-Like Factors 2 and 6 via RsmA-Mediated Regulation of Type III Exoenzymes S and Y. Infect. Immun., October 1, 2006; 74(10): 5893 - 5902. [Abstract] [Full Text] [PDF]

				K. L. Brigand, R. Russell, C. Moreilhon, J.-M. Rouillard, B. Jost, F. Amiot, V. Magnone, C. Bole-Feysot, P. Rostagno, V. Virolle, et al. An open-access long oligonucleotide microarray resource for analysis of the human and mouse transcriptomes Nucleic Acids Res., July 19, 2006; 34(12): e87 - e87. [Abstract] [Full Text] [PDF]

				S. Escotte, D. Al Alam, R. Le Naour, E. Puchelle, M. Guenounou, and S. C. Gangloff T Cell Chemotaxis and Chemokine Release after Staphylococcus aureus Interaction with Polarized Airway Epithelium Am. J. Respir. Cell Mol. Biol., March 1, 2006; 34(3): 348 - 354. [Abstract] [Full Text] [PDF]