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Physiol. Genomics 18: 79-86, 2004. First published March 9, 2004; doi:10.1152/physiolgenomics.00148.2003
1094-8341/04 $5.00
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Received 4 September 2003; accepted in final form 11 February 2004.
Physiological Genomics 18:79-86 (2004)
1094-8341/04 $5.00 © 2004 American Physiological Society

The mouse muscle creatine kinase promoter faithfully drives reporter gene expression in transgenic Xenopus laevis

Wayland Lim, Eric S. Neff and J. David Furlow

Section of Neurobiology, Physiology, and Behavior, Division of Biological Sciences, University of California, Davis, California 95616

Developing Xenopus laevis experience two periods of muscle differentiation, once during embryogenesis and again at metamorphosis. During metamorphosis, thyroid hormone induces both muscle growth in the limbs and muscle death in the tail. In mammals, the muscle creatine kinase (MCK) gene is activated during the differentiation from myoblasts to myocytes and has served as both a marker for muscle development and to drive transgene expression in transgenic mice. Transcriptional control elements are generally highly conserved throughout evolution, potentially allowing mouse promoter use in transgenic X. laevis. This paper compares endogenous X. laevis MCK gene expression and the mouse MCK (mMCK) promoter driving a green fluorescent protein reporter in transgenic X. laevis. The mMCK promoter demonstrated strong skeletal muscle-specific transgene expression in both the juvenile tadpole and adult frog. Therefore, our results clearly demonstrate the functional conservation of regulatory sequences in vertebrate muscle gene promoters and illustrate the utility of using X. laevis transgenesis for detailed comparative study of mammalian promoter activity in vivo.

transgenesis; frog; green fluorescent protein; gene expression




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