HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 18/1/51    most recent 00155.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Challacombe, J. F. Articles by Brettin, T. S. Search for Related Content PubMed PubMed Citation Articles by Challacombe, J. F. Articles by Brettin, T. S.

Evaluation of the host transcriptional response to human cytomegalovirus infection

¹ Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545
² Computing and Computational Sciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545
³ Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014

Gene expression data from human cytomegalovirus (HCMV)-infected cells were analyzed using DNA-Chip Analyzer (dChip) followed by singular value decomposition (SVD) and compared with a previous analysis of the same data that employed GeneChip software and a fold change filtering approach. dChip and SVD analysis revealed two clusters of coexpressed human genes responding differently to HCMV infection: one containing some genes identified previously, and another that was largely unique to this analysis. Annotating these genes, we identified several functional categories important to host cell responses to HCMV infection. These categories included genes involved in transcriptional regulation, oncogenesis, and cell cycle regulation, which were more prevalent in cluster 1, and genes involved in immune system regulation, signal transduction, and cell adhesion, which were more prevalent in cluster 2. Within these categories, we found genes involved in the host response to HCMV infection (mainly in cluster 1), as well as genes targeted by HCMV’s immune evasion strategies (mainly in cluster 2). As the second group of genes identified by the dChip and SVD approach was statistically and biologically significant, our results point out the advantages of using different methods to analyze gene expression data.

gene expression analysis; herpesvirus; pathogenesis

This article has been cited by other articles:

				C. Montag, J. Wagner, I. Gruska, and C. Hagemeier Human Cytomegalovirus Blocks Tumor Necrosis Factor Alpha- and Interleukin-1{beta}-Mediated NF-{kappa}B Signaling J. Virol., December 1, 2006; 80(23): 11686 - 11698. [Abstract] [Full Text] [PDF]

				M. Shlapobersky, R. Sanders, C. Clark, and D. H. Spector Repression of HMGA2 Gene Expression by Human Cytomegalovirus Involves the IE2 86-Kilodalton Protein and Is Necessary for Efficient Viral Replication and Inhibition of Cyclin A Transcription. J. Virol., October 1, 2006; 80(20): 9951 - 9961. [Abstract] [Full Text] [PDF]

				J. Sinclair and P. Sissons Latency and reactivation of human cytomegalovirus J. Gen. Virol., July 1, 2006; 87(7): 1763 - 1779. [Abstract] [Full Text] [PDF]

				J. Yuan, E. Cahir-McFarland, B. Zhao, and E. Kieff Virus and Cell RNAs Expressed during Epstein-Barr Virus Replication J. Virol., March 1, 2006; 80(5): 2548 - 2565. [Abstract] [Full Text] [PDF]

				S. A. K. Harvey, E. G. Romanowski, K. A. Yates, and Y. J. Gordon Adenovirus-Directed Ocular Innate Immunity: The Role of Conjunctival Defensin-like Chemokines (IP-10, I-TAC) and Phagocytic Human Defensin-{alpha} Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3657 - 3665. [Abstract] [Full Text] [PDF]