HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Physiol. Genomics 17: 11-20, 2004. First published December 16, 2003; doi:10.1152/physiolgenomics.00150.2003
1094-8341/04 $5.00

This Article Free upon publication Full Text Full Text (PDF) All Versions of this Article: 17/1/11    most recent 00150.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Mutch, D. M. Articles by Roberts, M.-A. Search for Related Content PubMed PubMed Citation Articles by Mutch, D. M. Articles by Roberts, M.-A.

Regional variations in ABC transporter expression along the mouse intestinal tract

¹ Nestlé Research Center, CH-1000 Lausanne 26
² Center for Integrative Genomics, Université de Lausanne, CH-1015 Lausanne
³ Swiss Institute for Experimental Cancer Research, 1066 Epalinges s/Lausanne, Switzerland
⁴ Nestle Purina Pet Care, St. Louis, Missouri 63164

The ATP-binding cassette (ABC) family of proteins comprise a group of membrane transporters involved in the transport of a wide variety of compounds, such as xenobiotics, vitamins, lipids, amino acids, and carbohydrates. Determining their regional expression patterns along the intestinal tract will further characterize their transport functions in the gut. The mRNA expression levels of murine ABC transporters in the duodenum, jejunum, ileum, and colon were examined using the Affymetrix MuU74v2 GeneChip set. Eight ABC transporters (Abcb2, Abcb3, Abcb9, Abcc3, Abcc6, Abcd1, Abcg5, and Abcg8) displayed significant differential gene expression along the intestinal tract, as determined by two statistical models (a global error assessment model and a classic ANOVA, both with a P < 0.01). Concordance with semiquantitative real-time PCR was high. Analyzing the promoters of the differentially expressed ABC transporters did not identify common transcriptional motifs between family members or with other genes; however, the expression profile for Abcb9 was highly correlated with fibulin-1, and both genes share a common complex promoter model involving the NFB, zinc binding protein factor (ZBPF), GC-box factors SP1/GC (SP1F), and early growth response factor (EGRF) transcription binding motifs. The cellular location of another of the differentially expressed ABC transporters, Abcc3, was examined by immunohistochemistry. Staining revealed that the protein is consistently expressed in the basolateral compartment of enterocytes along the anterior-posterior axis of the intestine. Furthermore, the intensity of the staining pattern is concordant with the expression profile. This agrees with previous findings in which the mRNA, protein, and transport function of Abcc3 were increased in the rat distal intestine. These data reveal regional differences in gene expression profiles along the intestinal tract and demonstrate that a complete understanding of intestinal ABC transporter function can only be achieved by examining the physiologically distinct regions of the gut.

ATP-binding cassette transporters; microarray; intestine; anterior-posterior axis

This article has been cited by other articles:

				D. M. Mutch, V. Crespy, J. Clough, C. J. Henderson, S. Lariani, R. Mansourian, J. Moulin, C. R. Wolf, and G. Williamson Hepatic cytochrome P-450 reductase-null mice show reduced transcriptional response to quercetin and reveal physiological homeostasis between jejunum and liver Am J Physiol Gastrointest Liver Physiol, July 1, 2006; 291(1): G63 - G72. [Abstract] [Full Text] [PDF]

				E. Viturro, C. Farke, H. H. D. Meyer, and C. Albrecht Identification, Sequence Analysis and mRNA Tissue Distribution of the Bovine Sterol Transporters ABCG5 and ABCG8 J Dairy Sci, February 1, 2006; 89(2): 553 - 561. [Abstract] [Full Text] [PDF]

				M. Marin-Kuan, S. Nestler, C. Verguet, C. Bezencon, D. Piguet, R. Mansourian, J. Holzwarth, M. Grigorov, T. Delatour, P. Mantle, et al. A Toxicogenomics Approach to Identify New Plausible Epigenetic Mechanisms of Ochratoxin A Carcinogenicity in Rat Toxicol. Sci., January 1, 2006; 89(1): 120 - 134. [Abstract] [Full Text] [PDF]