| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011
2 School of Biological Sciences, Washington State University, Pullman, Washington 99164
3 Department of Biology, University of California, Riverside, California 92521
4 Geriatric Research, Education and Clinical Center, W. S. Middleton Memorial Veterans Administration Hospital, Madison 53706
5 Department of Genetics and Medical Genetics, University of Wisconsin, Madison, Wisconsin 53706
6 Department of Medicine, University of Wisconsin, Madison, Wisconsin 53706
7 Department of Zoology, University of Wisconsin, Madison, Wisconsin 53706
We present the first quantitative gene expression analysis of cardiac aging under conditions of sedentary and active lifestyles using high-density oligonucleotide arrays representing 11,904 cDNAs and expressed sequence tags (ESTs). With these data, we test the hypothesis that exercise attenuates the gene expression changes that normally occur in the aging heart. Male mice (Mus domesticus) were sampled from the 16th generation of selective breeding for high voluntary exercise. For the selective breeding protocol, breeders were chosen based on the maximum number of wheel revolutions run on days 5 and 6 of a test at 8 wk of age. For the colony sampled herein, mice were housed individually over their entire lifetimes (from weaning) either with or without access to running wheels. The hearts of these two treatment groups (active and sedentary) were assayed at middle age (20 mo) and old age (33 mo). Genes significantly affected by age in the hearts of the sedentary population by at least a 50% expression change (n = 137) were distributed across several major categories, including inflammatory response, stress response, signal transduction, and energy metabolism. Genes significantly affected by age in the active population were fewer (n = 62). Of the 42 changes in gene expression that were common to both treatment groups, 32 (72%) displayed smaller fold changes as a result of exercise. Thus exercise offset many age-related gene expression changes observed in the hearts of the sedentary animals. These results suggest that adaptive physiological mechanisms that are induced by exercise can retard many effects of aging on heart muscle at the transcriptional level.
aging; artificial selection; exercise; microarray; Mus domesticus; stress/inflammation response
This article has been cited by other articles:
|
|
 |
|
  D. M. Huffman, D. R. Moellering, W. E. Grizzle, C. R. Stockard, M. S. Johnson, and T. R. Nagy Effect of exercise and calorie restriction on biomarkers of aging in mice Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1618 - R1627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Misra, H. Lee, A. Singh, K. Huang, R. K. Thimmulappa, W. Mitzner, S. Biswal, and C. G. Tankersley Global expression profiles from C57BL/6J and DBA/2J mouse lungs to determine aging-related genes Physiol Genomics, November 14, 2007; 31(3): 429 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Judge and C. Leeuwenburgh Cardiac mitochondrial bioenergetics, oxidative stress, and aging Am J Physiol Cell Physiol, June 1, 2007; 292(6): C1983 - C1992. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Finch A perspective on sporadic inclusion-body myositis: The role of aging and inflammatory processes Neurology, January 24, 2006; 66(1_suppl_1): S1 - S6. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Corton and H. M. Brown-Borg Peroxisome Proliferator-Activated Receptor {gamma} Coactivator 1 in Caloric Restriction and Other Models of Longevity J. Gerontol. A Biol. Sci. Med. Sci., December 1, 2005; 60(12): 1494 - 1509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Judge, Y. M. Jang, A. Smith, C. Selman, T. Phillips, J. R. Speakman, T. Hagen, and C. Leeuwenburgh Exercise by lifelong voluntary wheel running reduces subsarcolemmal and interfibrillar mitochondrial hydrogen peroxide production in the heart Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1564 - R1572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Kojda and R. Hambrecht Molecular mechanisms of vascular adaptations to exercise. Physical activity as an effective antioxidant therapy? Cardiovasc Res, August 1, 2005; 67(2): 187 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Melov and A. Hubbard Microarrays as a Tool to Investigate the Biology of Aging: A Retrospective and a Look to the Future Sci. Aging Knowl. Environ., October 20, 2004; 2004(42): re7 - re7. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Lennon, J. Quindry, K. L. Hamilton, J. French, J. Staib, J. L. Mehta, and S. K. Powers Loss of exercise-induced cardioprotection after cessation of exercise J Appl Physiol, April 1, 2004; 96(4): 1299 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Navarro, C. Gomez, J. M. Lopez-Cepero, and A. Boveris Beneficial effects of moderate exercise on mice aging: survival, behavior, oxidative stress, and mitochondrial electron transfer Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2004; 286(3): R505 - R511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J S. Pattison, L. C Folk, R. W Madsen, T. E Childs, E. E Spangenburg, and F. W Booth Expression profiling identifies dysregulation of myosin heavy chains IIb and IIx during limb immobilization in the soleus muscles of old rats J. Physiol., December 1, 2003; 553(2): 357 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ida, S. A. Boylan, A. L. Weigel, and L. M. Hjelmeland Age-related changes in the transcriptional profile of mouse RPE/choroid Physiol Genomics, November 11, 2003; 15(3): 258 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Welle and S. B. Glueck In for the long run: Focus on "Lifelong voluntary exercise in the mouse prevents age-related alterations in gene expression in the heart" Physiol Genomics, January 15, 2003; 12(2): 71 - 72. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
