HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 11/3/185    most recent 00077.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Peters, L. L. Articles by Bogue, M. A. Search for Related Content PubMed PubMed Citation Articles by Peters, L. L. Articles by Bogue, M. A.

Large-scale, high-throughput screening for coagulation and hematologic phenotypes in mice*

¹ The Jackson Laboratory, Bar Harbor, Maine 04609
² Dade Behring, Inc., Deerfield, Illinois

The Mouse Phenome Project is an international effort to systematically gather phenotypic data for a defined set of inbred mouse strains. For such large-scale projects the development of high-throughput screening protocols that allow multiple tests to be performed on a single mouse is essential. Here we report hematologic and coagulation data for more than 30 inbred strains. Complete blood counts were performed using an Advia 120 analyzer. For coagulation testing, we successfully adapted the Dade Behring BCS automated coagulation analyzer for use in mice by lowering sample and reagent volume requirements. Seven automated assay procedures were developed. Small sample volume requirements make it possible to perform multiple tests on a single animal without euthanasia, while reductions in reagent volume requirements reduce costs. The data show that considerable variation in many basic hematological and coagulation parameters exists among the inbred strains. These data, freely available on the World Wide Web, allow investigators to knowledgeably select the most appropriate strain(s) to meet their individual study designs and goals.

hemostasis; hematology; mouse phenome; NHLBI Program for Genomic Applications

This article has been cited by other articles:

				S. von Vietinghoff and K. Ley Homeostatic Regulation of Blood Neutrophil Counts J. Immunol., October 15, 2008; 181(8): 5183 - 5188. [Abstract] [Full Text] [PDF]

				M. J. Justice Removing the cloak of invisibility: phenotyping the mouse. Dis. Model. Mech., September 1, 2008; 1(2-3): 109 - 112. [Abstract] [Full Text] [PDF]

				S. B. Petkova, R. Yuan, S.-W. Tsaih, W. Schott, D. C. Roopenian, and B. Paigen Genetic influence on immune phenotype revealed strain-specific variations in peripheral blood lineages Physiol Genomics, August 1, 2008; 34(3): 304 - 314. [Abstract] [Full Text] [PDF]

				R. E. Rumbaut, R. V. Bellera, J. K. Randhawa, C. N. Shrimpton, S. K. Dasgupta, J.-F. Dong, and A. R. Burns Endotoxin enhances microvascular thrombosis in mouse cremaster venules via a TLR4-dependent, neutrophil-independent mechanism Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1671 - H1679. [Abstract] [Full Text] [PDF]

				S. Glueck Note from the Deputy Editor Physiol Genomics, December 3, 2002; 11(3): 109 - 109. [Full Text] [PDF]