Physiol. Genomics Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiol. Genomics (January 3, 2006). doi:10.1152/physiolgenomics.00254.2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Video and Table
Right arrow All Versions of this Article:
25/1/29    most recent
00254.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, J. C
Right arrow Articles by Quertermous, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, J. C
Right arrow Articles by Quertermous, T.
Submitted on October 14, 2005
Accepted on December 26, 2005

Transcriptional Profiling of Reporter Genes Used for Molecular Imaging of Embryonic Stem Cell Transplantation

Joseph C Wu1*, Joshua M Spin2, Feng Cao3, Shuan Lin3, Xiaoyan Xie3, Olivier Gheysens3, Ian Y Chen3, Ahmad Y Sheikh4, Robert C Robbins4, Anya Tsalenko5, Sanjiv S. Gambhir3, and Tom Quertermous2

1 Medicine/Cardiology Division, Stanford University School of Medicine, Stanford, CA, USA; Radiology and Bio-X Program, Stanford University School of Medicine, Stanford, CA, USA
2 Medicine/Cardiology Division, Stanford University School of Medicine, Stanford, CA, USA
3 Radiology and Bio-X Program, Stanford University School of Medicine, Stanford, CA, USA
4 Surgery, Stanford University School of Medicine, Stanford, CA, USA
5 Agilent Technologies, Palo Alto, CA, USA

* To whom correspondence should be addressed. E-mail: joewu{at}stanford.edu.

Stem cell therapy offers exciting promise for treatment of ischemic heart disease. Recent advances in molecular imaging techniques now allow investigators to monitor cell fate noninvasively and repetitively. Here we examine the effects of a triple fusion reporter gene on embryonic stem (ES) cell transcriptional profiles. Murine ES cells were stably transfected with a self-inactivating lentiviral vector carrying a triple fusion (TF) construct consisting of fluorescence, bioluminescence, and positron emission tomography (PET) reporter genes. Fluorescence activated cell sorting (FACS) analysis allowed isolation of stably transfected populations. Microarray studies comparing gene expression in nontransduced control ES cells versus stably transduced ES cells expressing triple fusion (ES-TF) revealed some increases in transcriptional variability. Annotation analysis showed that ES-TF cells down-regulated cell cycling, cell death, and protein and nucleic acid metabolism genes, while upregulating homeostatic and anti-apoptosis genes. Despite these transcriptional changes, expression of the TF reporter gene had no significant effects on ES cell viability, proliferation, and differentiation capability. Importantly, transplantation studies in murine myocardium demonstrated the feasibility of tracking ES-TF cells in living subjects using bioluminescence and PET imaging. Taken together, this is the first study to analyze in detail the effects of reporter genes on molecular imaging of ES cells.




This article has been cited by other articles:


Home page
 JNMHome page
T. Higuchi, M. Anton, K. Dumler, S. Seidl, J. Pelisek, A. Saraste, A. Welling, F. Hofmann, R. A.J. Oostendorp, B. Gansbacher, et al.
Combined Reporter Gene PET and Iron Oxide MRI for Monitoring Survival and Localization of Transplanted Cells in the Rat Heart
J. Nucl. Med., July 1, 2009; 50(7): 1088 - 1094.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
F. Wang, J. E. Dennis, A. Awadallah, L. A. Solchaga, J. Molter, Y. Kuang, N. Salem, Y. Lin, H. Tian, J. A. Kolthammer, et al.
Transcriptional profiling of human mesenchymal stem cells transduced with reporter genes for imaging
Physiol Genomics, March 3, 2009; 37(1): 23 - 34.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
J. C. Wu
Molecular Imaging: Antidote to Cardiac Stem Cell Controversy
J. Am. Coll. Cardiol., November 11, 2008; 52(20): 1661 - 1664.
[Full Text] [PDF]

Home page
 Circ Cardiovasc ImagingHome page
M. Gyongyosi, J. Blanco, T. Marian, L. Tron, O. Petnehazy, Z. Petrasi, R. Hemetsberger, J. Rodriguez, G. Font, I. J. Pavo, et al.
Serial Noninvasive In Vivo Positron Emission Tomographic Tracking of Percutaneously Intramyocardially Injected Autologous Porcine Mesenchymal Stem Cells Modified for Transgene Reporter Gene Expression
Circ Cardiovasc Imaging, September 1, 2008; 1(2): 94 - 103.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
Z. Lee, J. E. Dennis, and S. L. Gerson
Imaging Stem Cell Implant for Cellular-Based Therapies
Experimental Biology and Medicine, August 1, 2008; 233(8): 930 - 940.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
Z. Love, F. Wang, J. Dennis, A. Awadallah, N. Salem, Y. Lin, A. Weisenberger, S. Majewski, S. Gerson, and Z. Lee
Imaging of Mesenchymal Stem Cell Transplant by Bioluminescence and PET
J. Nucl. Med., December 1, 2007; 48(12): 2011 - 2020.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
Z. Li, J. C. Wu, A. Y. Sheikh, D. Kraft, F. Cao, X. Xie, M. Patel, S. S. Gambhir, R. C. Robbins, J. P. Cooke, et al.
Differentiation, Survival, and Function of Embryonic Stem Cell Derived Endothelial Cells for Ischemic Heart Disease
Circulation, September 11, 2007; 116(11_suppl): I-46 - I-54.
[Abstract] [Full Text] [PDF]

Home page
 RadiologyHome page
J. C. Wu, F. M. Bengel, and S. S. Gambhir
Cardiovascular Molecular Imaging
Radiology, August 1, 2007; 244(2): 337 - 355.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.