Submitted on September 21, 2005
Accepted on February 22, 2006
qPCR-DAMS: a Database Tool to Analyze, Manage, and Store Both Relative and Absolute Quantitative Real-Time PCR data
Nili Jin1, Keyu He1, and Lin Liu1*
1 Physiological Sciences, Oklahoma State University, Stillwater, OK, USA
* To whom correspondence should be addressed. E-mail: lin.liu{at}okstate.edu.
Quantitative real-time PCR is an important high throughput method in biomedical sciences. However, existing software has limitations in handling both relative and absolute quantification. We designed qPCR-DAMS (Quantitative PCR Data Analysis and Management System), a database tool based on Access 2003, to deal with such shortcomings by the addition of integrated mathematical procedures. qPCR-DAMA allows a user choose among four methods for data processing within a single software package: (I) Ratio relative quantification, (II) Absolute level, (III) Normalized absolute expression, and (IV) Ratio absolute quantification. qPCR-DAMS also provides a tool for multiple reference gene normalization. qPCR-DAMS has three quality control steps and a data display system to monitor data variation. In summary, qPCR-DAMS is a handy tool for real-time PCR users.
Availability: This software is free for academic use and downloadable at
http://www.cvm.okstate.edu/research/Facilities/LungBiologyLab/
Contact: lin.liu@okstate.edu
