| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Medicine, Lung Translational Genomics Center, Pittsburgh, PA, USA
2 Institute for Human and Machine Cognition, University of West Florida, Pensacola, FL, USA
* To whom correspondence should be addressed. E-mail: dgp{at}imap.pitt.edu.
We have utilized Serial Analysis of Gene Expression (SAGE) to analyze the temporal response of human aortic endothelial cells (HAECs) to short-term chronic hypoxia at the level of transcription. Primary cultures of HAECs were exposed to 1% O2 hypoxia for 8 and 24 hours and compared to identical same passage cells cultured under standard (5% CO2 95% air) conditions. A total of 121446 tags representing 37096 unique tags were sequenced and genes whose expression levels were modulated by hypoxia identified by novel statistical analyses. Hierarchical clustering of genes displaying statistically significant hypoxia-responsive alterations in expression revealed temporal modulation of a number of major functional gene families including those encoding heat shock factors, glycolytic enzymes, extracellular matrix factors, cytoskeletal factors, apoptotic factors, cell cycle regulators and angiogenic factors. Within these families we documented the coordinated modulation of both previously known hypoxia-responsive genes, numerous genes whose expressions have not been previously shown to be altered by hypoxia, tags matching uncharacterized UniGene entries and entirely novel tags with no UniGene match. These preliminary data, which indicate a reduction in cell cycle progression, elevated metabolic stress and increased cytoskeletal remodeling under acute hypoxic stress, provide a foundation for further analyses of the molecular mechanisms underlying the endothelial response to short-term chronic hypoxia.
This article has been cited by other articles:
|
|
 |
|
  W. Ning, Y. Dong, J. Sun, C. Li, M. A. Matthay, C. A. Feghali-Bostwick, and A. M. K. Choi Cigarette Smoke Stimulates Matrix Metalloproteinase-2 Activity via EGR-1 in Human Lung Fibroblasts Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 480 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Degrossoli and S. Giorgio Functional Alterations in Macrophages After Hypoxia Selection Experimental Biology and Medicine, January 1, 2007; 232(1): 88 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Peters, W. Ning, T. J. Chu, C. J. Li, and A. M. K. Choi Comparative SAGE analysis of the response to hypoxia in human pulmonary and aortic endothelial cells Physiol Genomics, September 14, 2006; 26(2): 99 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Dolinay, N. Kaminski, M. Felgendreher, H. P. Kim, P. Reynolds, S. C. Watkins, D. Karp, S. Uhlig, and A. M. K. Choi Gene expression profiling of target genes in ventilator-induced lung injury Physiol Genomics, September 14, 2006; 26(1): 68 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bertl, H. Bartsch, and C. Gerhauser Inhibition of angiogenesis and endothelial cell functions are novel sulforaphane-mediated mechanisms in chemoprevention. Mol. Cancer Ther., March 1, 2006; 5(3): 575 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Williams and W. J. Pearce Age-dependent modulation of endothelium-dependent vasodilatation by chronic hypoxia in ovine cranial arteries J Appl Physiol, January 1, 2006; 100(1): 225 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. M. van der Meer, G. E. E. J. M. van den Thillart, F. Witte, M. A. G. de Bakker, J. Besser, M. K. Richardson, H. P. Spaink, J. T. D. Leito, and C. P. Bagowski Gene expression profiling of the long-term adaptive response to hypoxia in the gills of adult zebrafish Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2005; 289(5): R1512 - R1519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mondon, T.-M. Mignot, R. Rebourcet, H. Jammes, J.-L. Danan, F. Ferre, and D. Vaiman Profiling of oxygen-modulated gene expression in early human placenta by systematic sequencing of suppressive subtractive hybridization products Physiol Genomics, June 16, 2005; 22(1): 99 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ning, C.-J. Li, N. Kaminski, C. A. Feghali-Bostwick, S. M. Alber, Y. P. Di, S. L. Otterbein, R. Song, S. Hayashi, Z. Zhou, et al. Comprehensive gene expression profiles reveal pathways related to the pathogenesis of chronic obstructive pulmonary disease PNAS, October 12, 2004; 101(41): 14895 - 14900. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
