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1 Equipe INSERM-ESPRI/EA-3855, Faculty of Medicine, University François Rabelais, Tours, France
2 Service de Génomique Fonctionnelle, CEA, Evry, France
3 CECS/1-STEM, Evry, France
4 Equipe INSERM-ESPRI/EA-3855, Faculty of Medicin, University François Rabelais, Tours, France; EFS Centre-Atlantiique, Tours, France
5 Equipe INSERM-ESPRI/EA-3855, Faculté de médecine, Université François Rabelais, Tours, France
6 Equipe INSERM-ESPRI/EA-3855, Faculté de médecine, Université François Rabelais, Tours, France; EFS Centre-Atlantiique, Tours, France
7 Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States
* To whom correspondence should be addressed. E-mail: charbord{at}med.univ-tours.fr.
We determined a transcriptional profile specific for clonal stromal mesenchymal stem cells from adult and fetal hematopoietic sites. To identify mesenchymal stem cell-like stromal cell lines we evaluated the adipocytic (A), osteoblastic (O), chondrocytic (C), and vascular smooth muscle (V) differentiation potential, and also the hematopoietic supportive (stromal) capacity of 6 mouse stromal cell lines from adult bone marrow and day 14.5 fetal liver. We found that 2 lines were quadripotent and also supported hematopoiesis, BMC9 from bone marrow, and AFT024 from fetal liver. We then ascertained the set of genes differentially expressed in the intersection set of AFT024 and BMC9 as compared to those expressed in the union set of 2 negative control lines, 2018 and BFC012 (both from fetal liver); 346 genes were up-regulated and 299 down-regulated. Using Ingenuity software, we found 2 major gene networks with highly significant scores. One network contained down-regulated genes that are known to be implicated in osteoblastic differentiation, proliferation or transformation. The other network contained up-regulated genes which belonged to 2 categories, cytoskeletal genes and genes implicated in the transcriptional machinery. The data extend the concept of stromal mesenchymal stem cells to clonal cell populations derived not only from bone marrow, but also from fetal liver. The gene networks described should discriminate this cell type from other types of stem cells and help define the stem cell state.
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