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Physiol. Genomics (February 3, 2004). doi:10.1152/physiolgenomics.00186.2003
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Submitted on November 3, 2003
Accepted on January 29, 2004

A Comprehensive Non-redundant Expressed Sequence Tag Collection for the Developing Rattus norvegicus Heart

Jennifer J. S Laffin1*, Todd E Scheetz2, Maria de Fatima Bonaldo1, Rebecca S Reiter3, Shereen Chang1, Mari Eyestone1, Hakeem Abdulkawy2, Bartley Brown2, Chad Roberts2, Dylan Tack2, Tamara Kucaba1, Jim Jung-Ching Lin3, Val C Sheffield1, Thomas L Casavant2, and M. Bento Soares4

1 Pediatrics and Interdepartmental-Genetics Graduate Program, University of Iowa, Iowa City, IA, USA
2 Engineering, University of Iowa, Iowa City, IA, USA
3 Biological Sciences, University of Iowa, Iowa City, IA, USA
4 Pediatrics and Interdepartmental-Genetics Graduate Program, University of Iowa, Iowa City, IA, USA; Biochemistry, Physiology and Biophysics, and Orthopaedics, University of Iowa, Iowa City, IA, USA

* To whom correspondence should be addressed. E-mail: jennifer-laffin{at}uiowa.edu.

Congenital heart defects affect approximately 1,000,000 people in the United States, with 40,000 new births contributing to that number every year. A large percentage of these defects can be attributed to septal defects. We assembled a non-redundant collection of over 12,000 Expressed Sequence Tags (ESTs) from a total of 30,000 ESTs, with the ultimate goal of identifying spatially and/or temporally regulated genes during heart septation. These ESTs were compiled from non-normalized, normalized and serially subtracted cDNA libraries derived from two sets of tissue samples. The first includes micro dissected rat hearts from embryonic days 13, 15 and 16.5-18.5, and adult heart. The second includes hearts from embryonic days 17, 19, 21, and post-natal days 1,12,74, and 200. Over 6,000 novel ESTs were identified in the libraries derived from these two sets of tissues, all of which have been contributed to the NCBI rat Unigene collection. It is anticipated that such EST and cDNA clone resources will prove invaluable to gene expression studies aimed at the understanding of the molecular mechanisms underlying heart septation defects.




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