Physiol. Genomics Journal of Neurophysiology
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Physiol. Genomics (December 12, 2006). doi:10.1152/physiolgenomics.00167.2006
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Submitted on July 28, 2006
Accepted on December 1, 2006

Responses of the Human Airway Epithelium Transcriptome to In Vivo Injury

Adriana Heguy1, Ben-Gary Harvey1, Philip L Leopold1, Igor Dolgalev1, Tina Raman1, and Ronald G. Crystal1*

1 Genetic Medicine, Weill Medical College of Cornell University, New York, New York, United States

* To whom correspondence should be addressed. E-mail: geneticmedicine{at}med.cornell.edu.

To identify genes participating in human airway epithelial repair, we used bronchoscopy and brushing to denude the airway epithelium of healthy individuals, sequentially sampled the same region 7 and 14 days later, and assessed gene expression by Affymetrix microarrays with TaqMan RT-PCR confirmation. Histologically, the injured area was completely covered by a partially re-differentiated epithelial layer after 7 days; by 14 days the airway epithelium was very similar to the uninjured state. At day 7 compared to resting epithelium, there were substantial differences in gene expression pattern, with a distinctive airway epithelial repair transcriptome of actively proliferating cells in the process of re-differentiation. The repair transcriptome at 7 days was dominated by cell cycle, signal transduction, metabolism and transport and transcription genes. Interestingly, the majority of differentially expressed cell cycle genes belonged to the G2 and M phases, suggesting that the proliferating cells are relatively synchronized 1 wk following injury. At 14 days post-injury, the expression profile was similar to that of resting airway epithelium. These observations provide a baseline of the functional gene categories participating in the process of normal human airway epithelial repair that can be used in future studies of injury and repair in airway epithelial diseases.




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