Physiol. Genomics AJP: Gastrointestinal and Liver Physiology
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Physiol. Genomics (July 20, 2005). doi:10.1152/physiolgenomics.00141.2004
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Submitted on June 15, 2004
Accepted on July 14, 2005

Global transcriptional characterization of SP and MP cells from the myogenic C2C12 cell line : effect of FGF6

Charles Decraene1, Rachid Benchaouir2, Marie-Agnes Dillies1, David Israeli2, Sylvie Bortoli1, Christelle Rochon1, Philippe Rameau2, Amandine Pitaval1, Diana Tronik-Le Roux1, Olivier Danos2, Xavier Gidrol1, Luis Garcia2, and Genevieve Pietu1*

1 Service de Genomique Fonctionnelle, CEA, Evry Cedex, France
2 Centre National de la Recherche Scientifique, GENETHON, Evry Cedex, France

* To whom correspondence should be addressed. E-mail: gpietu{at}istem.genethon.fr.

By using Hoechst staining techniques, we have previously shown that the C2C12 myogenic cell line contains a Side Population (SP) which is largely increased in the presence of FGF6. Here, we compared transcriptional profiles from SP and MP (Main Population) cells from either C2C12 or FGF6-expressing C2C12. Expression profiles of SPs show that these cells are less differentiated than MPs and display some similarities to stem cells. Moreover, Principal Component Analysis (PCA) makes it possible to distinguish specific contributions of either FGF6 or differentiation effects on gene expression profiles. This demonstrated that FGF6-expanded SPs were similar to the parental C2C12-derived SPs. Conversely, FGF6-treated MPs differed from parental MPs and were more related to SP cells. These results show that FGF6 pushed committed myogenic cells towards a more immature phenotype resulting in the accumulation of cells with a SP phenotype. We propose that FGF6 conditioning could provide a way to expand the pool of immature cells by myoblast dedifferentiation.




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