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1 Laboratory of Molecular Genetics, G.Gaslini Institute, Genova, Italy
2 Epidemiology and Statistics Unit, G.Gaslini Institute, Genova, Italy
3 Laboratory of Nephrology, G.Gaslini Institute, Genova, Italy
4 Laboratory of Chemical-Clinical Analysis, G.Gaslini Institute, Genova, Italy
5 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
6 Laboratory of Molecular Genetics, G.Gaslini Institute, Genova, Italy; Department of Pediatrics and CEBR, University of Genova, Genova, Italy
* To whom correspondence should be addressed. E-mail: rravazzo{at}unige.it.
Understanding the molecular mechanisms that underly regulation of transcription of the human Osteopontin encoding gene (OPN) may help to clarify several processes, such as fibrotic evolution of organ damage, tumorigenesis and metastasis, and immune response, in which OPN overexpression is observed. With the aim to evaluate variants with functional effect on transcription, we have analyzed the promoter region and focused our investigation on three common variants present in the first 500 bp upstream of the transcription start site. Transfection of constructs carrying the four most frequent haplotypes relative to variants at -66, -156 and -443 fused to the luciferase reporter gene in a panel of different cell lines, showed that one haplotype conferred a significantly reduced level of reporter gene expression in all tested cell lines. We describe that the -66 polymorphism modifies the binding affinity for the SP1/SP3 transcription factors, the -156 polymorphism is included in a yet uncharacterized RUNX2 binding site and the -443 polymorphism causes differential binding of an unknown factor. The finding of differential effects of various combinantion of variants in haplotypes may contribute to explain data of association studies reported in several already published articles. Future association studies using haplotypes instead of single OPN variants will allow to achieve more accurate results referrable to differential expression of OPN in several common diseases, in which OPN is considered a candidate susceptibility gene.
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