| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany
2 Department of Animal Sciences, Laval University, Centre de Recherche en Biologie de la Reproduction, Quebec, Canada
* To whom correspondence should be addressed. E-mail: tesfaye{at}itz.uni-bonn.de.
The purpose of this work is to address the relationship between transcriptional profile of embryos and the pregnancy success based on gene expression analysis of blastocyst biopsies taken prior to transfer to recipients. Biopsies (30-40% of the intact embryo) were taken from IVP day 7 blastocysts (n=118) and 60-70% part were transferred to recipients after re-expansion. Based on the success of pregnancy, biopsies were pooled in three groups (each 10 biopsies) namely: those resulted in no pregnancy (G1), resorbed embryos (G2) and those resulted in calf delivery (G3). Gene expression analysis of these groups was performed using home made bovine preimplantation specific cDNA array (219 clones) and BlueChip (with ~2000 clones). Microarray data analysis results revealed a total of 52 and 58 genes were differentially regulated during comparison between G1 versus G3 and G2 versus G3. Biopsies resulted in calf delivery were enriched with genes necessary for implantation (COX2 and CDX2), carbohydrate metabolism (ALOX15), growth factor (BMP15), signal transduction (PLAU) and placenta-specific 8 (PLAC8). Biopsies from embryos resulted in resorption are enriched with transcripts involved protein phosphorylation (KRT8), Plasma membrane (OCLN) and glucose metabolism (PGK1 & AKR1B1). Biopsies from embryos resulted in no pregnancy are enriched with transcripts involved inflammatory cytokines (TNF), protein amino acid binding (EEF1A1), transcription factors (MSX1, PTTG1), glucose metabolism (PGK1, AKR1B1) and CD9 which is inhibitor of implantation. In conclusion, we generated direct candidates of blastocyst specific genes which may play an important role in determining the fate of the embryo after transfer.
This article has been cited by other articles:
|
|
 |
|
  S L Rodriguez-Zas, K Schellander, and H A Lewin Biological interpretations of transcriptomic profiles in mammalian oocytes and embryos Reproduction, February 1, 2008; 135(2): 129 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Duranthon, A. J Watson, and P. Lonergan Preimplantation embryo programming: transcription, epigenetics, and culture environment Reproduction, February 1, 2008; 135(2): 141 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Torner, N. Ghanem, C. Ambros, M. Holker, W. Tomek, C. Phatsara, H. Alm, M.-A. Sirard, W. Kanitz, K. Schellander, et al. Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity Reproduction, February 1, 2008; 135(2): 197 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Burton and G. J. M. Rosa Physiological genomics special issue on animal functional genomics Physiol Genomics, December 13, 2006; 28(1): 1 - 4. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
