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Physiol. Genomics (October 21, 2003). doi:10.1152/physiolgenomics.00108.2003
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Submitted on July 7, 2003
Accepted on October 10, 2003

SERIAL ANALYSIS OF GENE EXPRESSION IN MOUSE KIDNEY FOLLOWING ANGIOTENSIN II ADMINISTRATION

Faina Schwartz1*, Arvi Duka1, Elena Triantafyllidi1, Conrado Johns1, Irena Duka1, Jing Cui1, and Haralambos Gavras1

1 Medicine, Hypertension Section, Boston University School of Medicine, Boston, MA, USA

* To whom correspondence should be addressed. E-mail: fschw{at}bu.edu.

As a new line of inquiry into the molecular mechanisms underlying pathophysiological processes associated with angiotensin (AngII) dependent hypertension, we applied the method of Serial Analysis of Gene Expression (SAGE) to examine genome-wide transcription changes in the kidneys of mice that developed hypertension in response to chronic AngII administration. Mice were infused subcutaneously via osmotic minipumps with AngII for 7 days, and systolic blood pressure measured by tail-cuff plethysmography. Subsequently, mice were euthanized, and the total RNA isolated from the kidneys was used to construct SAGE libraries. Comparison of 11,447 SAGE tags from the hypertensive kidneys, representing 5740 unique transcripts, and 11,273 tags from the control kidneys, corresponding to 5619 different transcripts, identified genes that are significantly (p<=0.05) down- or up-regulated in the hypertensive kidney. Our assessment of the genome-wide influence of AngII resulted in the detection of several novel genes and in a recognition of potential new roles for the previously characterized genes, thus providing new probes with which to further explore the AngII effects in normal and disease states.




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