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Articles in PresS, published online ahead of print August 27, 2002
Physiol Genomics, 10.1152/physiolgenomics.00108.2001
Submitted on November 13, 2001
Accepted on August 23, 2002
1 Department of Animal Sciences, Purdue University, West Lafayette, IN, USA
* To whom correspondence should be addressed. E-mail: sdonkin{at}purdue.edu.
The cytosolic (C) and mitochondrial (M) forms of phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.32) are encoded by two different nuclear genes in mouse, human, and chicken. Our objective was to clone the two forms of PEPCK for bovine and determine their expression during the immediate periparturient interval in dairy cows. Bovine PEPCK-C cDNA contains 2592 nucleotides and contains 84% similarity to the coding sequence of human PEPCK-C cDNA. A 449 nt partial clone of the 3' end of PEPCK-M is 76% similar to the corresponding sequence of human PEPCK-M. The coding sequence of bovine PEPCK-C and coding sequence of the partial PEPCK-M clone were 58% similar but the similarities in the 3' untranslated regions were negligible. Northern blot analysis revealed single transcripts of 2.85 and 2.35 kb for PEPCK-C and PEPCK-M, respectively. The transition to lactation did not alter PEPCK-M transcript expression but expression of PEPCK-C mRNA was transiently increased during early lactation indicating that enhanced hepatic gluconeogenesis during this period may be tied to enhanced capacity for cytosolic rather than mitochondrial formation of phosphoenolpyruvate.
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