Physiol. Genomics Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiol. Genomics (October 14, 2003). doi:10.1152/physiolgenomics.00099.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
16/1/153    most recent
00099.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nobis, W.
Right arrow Articles by Coussens, P. M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nobis, W.
Right arrow Articles by Coussens, P. M
Submitted on June 13, 2003
Accepted on September 29, 2003

DEVELOPMENT OF A PORCINE BRAIN CDNA LIBRARY, EST DATABASE AND MICROARRAY RESOURCE

William Nobis1, Xiaoning Ren1, Steven P Suchyta1, Thomas R Suchyta2, Adroaldo J Zanella3, and Paul M Coussens1*

1 Animal Science, Center for Animal Functional Genomics Michigan State University, East Lansing, MI, USA
2 Animal Science, Animal Behavior and Welfare Group, Michigan State University, East Lansing, MI, USA
3 Texas Instruments, Stafford, TX, USA

* To whom correspondence should be addressed. E-mail: coussens{at}msu.edu.

Recent developments in expressed sequence tag (EST) and cDNA microarray technology have had a dramatic impact on the ability of scientists to study responses of thousands of genes to internal and external stimuli. In neurobiology, studies of the human brain have been expanding rapidly by use of functional genomics techniques. To enhance these studies and allow use of a porcine brain model, a normalized porcine brain cDNA library has been generated and used as a base for EST discovery and microarray generation. In this report, we discuss initial sequence analysis of 965 clones from this resource. Our data revealed that library normalization successfully reduced the number of clones representing highly abundant cDNA species and overall clone redundancy. Cluster analysis revealed over 800 unique cDNA species representing a redundancy rate for the normalized library of 6.9% compared to 29.4% before normalization. Sequence information, BLAST results, and TIGR cluster matches for these ESTs are publicly available via a web-accessible database (http://nbfgc.msu.edu). A cDNA microarray was created using 877 unique porcine brain EST amplicons spotted in triplicate on glass slides. This microarray was assessed by performing a series of experiments designed to test hybridization efficiency and false positive rate. Our results indicate that the porcine brain (PBL) cDNA microarray is a robust tool for studies of brain gene expression using swine as a model system.




This article has been cited by other articles:


Home page
 Brief Funct Genomic ProteomicHome page
R. Davoli and S. Braglia
Molecular approaches in pig breeding to improve meat quality
Brief Funct Genomic Proteomic, January 21, 2008; (2008) elm036v1.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A. Reverter, N. J. Hudson, Y. Wang, S.-H. Tan, W. Barris, K. A. Byrne, S. M. McWilliam, C. D. K. Bottema, A. Kister, P. L. Greenwood, et al.
A gene coexpression network for bovine skeletal muscle inferred from microarray data
Physiol Genomics, December 13, 2006; 28(1): 76 - 83.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. Liang and B. Ventura
Physiological genomics in PG and beyond: October to December 2005
Physiol Genomics, December 14, 2005; 24(1): 1 - 3.
[Full Text] [PDF]

Home page
 Vet PatholHome page
P. R. Nambiar, S. R. Boutin, R. Raja, and D. W. Rosenberg
Global Gene Expression Profiling: A Complement to Conventional Histopathologic Analysis of Neoplasia
Vet. Pathol., November 1, 2005; 42(6): 735 - 752.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.