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Physiol. Genomics (June 5, 2002). doi:10.1152/physiolgenomics.00095.2001
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Articles in PresS, published online ahead of print June 5, 2002
Physiol Genomics, 10.1152/physiolgenomics.00095.2001
Submitted on October 2, 2001
Accepted on May 30, 2002

Differential Expression of the NAT1 Translational Repressor During Development of Bovine Intramuscular Adipocytes

Kirby D. Childs1, David W. Goad2, Mark F. Allan3, Daniel Pomp3, Clinton Krehbiel1, Rodney D. Geisert1, James B. Morgan1, and Jerry R. Malayer2*

1 Animal Sciences, Oklahoma State University, Stillwater, OK, USA
2 Physiological Sciences, Oklahoma State University, Stillwater, OK, USA
3 Animal Sciences, University of Nebraska, Lincoln, NE, USA

* To whom correspondence should be addressed. E-mail: malayer{at}okstate.edu.

This study was undertaken to test for differential gene expression in intramuscular adipocytes during fat deposition of feedlot steers. Angus X Hereford steers (n=50) were fed a high-energy concentrate ration ad libitum for 20 d (n=5), 86 d (n=15), 121 d (n=15) and 146 d (n=15) to obtain various degrees of intramuscular adipocyte development. Carcass traits were significantly different (p<.05) between the groups. Intramuscular adipose was excised from the longissimus dorsi and snap frozen in liquid nitrogen. Pooled samples of total RNA representing each group were analyzed by differential-display polymerase chain reaction (ddPCR) using 200 primer combinations comprised of 20 arbitrary (5') and 10 anchor (3') oligonucleotides. Bands (n=70) representing putative differences among treatment groups were excised, sequenced and subjected to BLAST homology search. From these, 40 contained significant homology to known genes. One was of particular interest, the translational repressor NAT1 (Novel APOBEC-1 Target-1). NAT1 mRNA was quantified in individual animals to confirm differential expression among treatment groups. Results indicate that NAT1 message is more abundant (p<.05) in intramuscular adipocytes of younger/leaner animals.




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