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1 Animal Science, Michigan State University, East Lansing, Michigan, USA; Center for Animal Functional Genomics, Michigan State University, East Lansing, Michigan, USA
2 Center for Animal Functional Genomics, Michigan State University, East Lansing, Michigan, USA
3 Genomics Technology Support Facility, Michigan State University, East Lansing, Michigan, USA
4 Animal Science, Cornell University, Ithaca, New York, USA
5 Animal Science, University of Arizona, Tucson, Arizona, USA
6 Animal Science, University of California, Davis, California, USA
7 Animal Science, University of Missouri, Columbia, Missouri, USA
8 Animal and Veterinary Science, University of Idaho, Moscow, Idaho, USA
9 Agricultural Research Service, United States Meat Animal Research Center, United States Department of Agriculture, Clay Center, Nebraska, USA
10 Agricultural Research Service, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland, USA
* To whom correspondence should be addressed. E-mail: suchytas{at}msu.edu.
A cDNA microarray resource has been developed with the goal of providing integrated functional genomics resources for cattle. The National Bovine Functional Genomics Consortium's (NBFGC) EST collection was established in 2001 to develop resources for functional genomics research. The NBFGC EST collection and microarray contains 18,263 unique transcripts, derived from many different tissue types and various physiologically important states within these tissues. The NBFGC microarray has been tested for false positive rates using self-self hybridizations and was shown to yield robust results in test microarray experiments. A web-accessible database has been established to provide pertinent data related to NBFGC clones, including sequence data, BLAST results, and ontology information. The NBFGC microarray represents the largest cDNA microarray for a livestock species prepared to date and should prove to be a valuable tool in studying genome-wide gene expression in cattle.
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