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Physiol. Genomics (July 12, 2005). doi:10.1152/physiolgenomics.00080.2005
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Submitted on April 4, 2005
Accepted on June 16, 2005

Variation in gene expression profiles of peripheral blood mononuclear cells from healthy volunteers

John J Eady1, Gary M Wortley1, Yvette M Wormstone1, Jackie C Hughes1, Sian B Astley1, Robert J Foxall1, Joanne F Doleman1, and Ruan M Elliott1*

1 Institute of Food Research, Norwich, Norfolk, United Kingdom

* To whom correspondence should be addressed. E-mail: ruan.elliott{at}bbsrc.ac.uk.

The normal degree of intra- and inter-individual variation in gene transcription profiles of healthy human tissues has not been extensively investigated. In the study described here, microarrays were employed to analyse gene transcription in peripheral blood mononuclear cells prepared from serial blood samples that had been obtained, at weekly intervals, from apparently healthy human volunteers. Transcript levels for the majority of genes examined were found to be remarkably consistent within samples from a single donor. Conversely, marked differences were observed in samples obtained from different donors. Genes that exhibited differential expression dependent on sex, age, body mass index and the presence of varying proportions of different leucocyte subsets were identified. These results emphasise the important contributions of genetic and environmental factors, as well as varying representation of different cell types, in determining the overall gene transcriptional profiles of human tissues. However, the study also provides evidence that, within an individual, the gene transcription profiles of sampled tissues can be comparatively stable over time.




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