Physiol. Genomics Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiol. Genomics (August 2, 2005). doi:10.1152/physiolgenomics.00076.2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Tables
Right arrow All Versions of this Article:
23/2/227    most recent
00076.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brauch, K. M
Right arrow Articles by Andrews, M. T
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brauch, K. M
Right arrow Articles by Andrews, M. T
Submitted on March 31, 2005
Accepted on July 25, 2005

Digital transcriptome analysis indicates adaptive mechanisms in the heart of a hibernating mammal

Katharine M Brauch1, Nirish D Dhruv2, Eric A Hanse1, and Matthew T Andrews1*

1 Biology, University of Minnesota Duluth, Duluth, Minnesota, USA
2 Computer Science, University of Minnesota Duluth, Duluth, Minnesota, USA

* To whom correspondence should be addressed. E-mail: mandrews{at}d.umn.edu.

Survival of near freezing body temperatures and reduced blood flow during hibernation is likely the result of changes in the expression of specific genes. In this paper we describe a comprehensive survey of mRNAs in the heart of the thirteen-lined ground squirrel (Spermophilus tridecemlineatus) before and during hibernation. The heart was chosen for this study because it is a contractile organ that must continue to work despite body temperatures of 5°C and the lack of food for periods of 5-6 months. We used a digital gene expression assay involving high-throughput sequencing of directional cDNA libraries from hearts of active and hibernating ground squirrels to determine the identity and frequency of 3,532 expressed sequence tags (ESTs). Statistical analysis of the active and hibernating heart expression profile indicated the differential regulation of 48 genes based on a p ≤ 0.03 threshold. Several of the differentially expressed genes identified in this screen encode proteins that likely account for uninterrupted cardiac function during hibernation including those involved in metabolism, contractility, Ca2+ handling and low temperature catalysis. A sampling of genes showing higher expression during hibernation include phosphofructokinase (PFK), pancreatic triacylglycerol lipase (PTL), pyruvate dehydrogenase kinase 4 (PDK4), aldolase A, sarco(endo)plasmic reticulum calcium-ATPase 2a (SERCA2a), titin, and four and a half LIM domains protein 2 (FHL2). Genes showing reduced levels of expression during hibernation include CDK2-associated protein 1 (CDK2AP1), troponin C, phospholamban (PLB), calcium-calmodulin dependent protein kinase II (CaMKII), calmodulin (CaM), and four subunits of cytochrome c oxidase.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Miyazawa, Y. Shimizu, T. Shiina, H. Hirayama, H. Morita, and T. Takewaki
Central A1-receptor activation associated with onset of torpor protects the heart against low temperature in the Syrian hamster
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2008; 295(3): R991 - R996.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
O. L. Nelson, C. T. Robbins, Y. Wu, and H. Granzier
Titin isoform switching is a major cardiac adaptive response in hibernating grizzly bears
Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H366 - H371.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. Ruf and W. Arnold
Effects of polyunsaturated fatty acids on hibernation and torpor: a review and hypothesis
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R1044 - R1052.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Yan, B. M. Barnes, F. Kohl, and T. G. Marr
Modulation of gene expression in hibernating arctic ground squirrels
Physiol Genomics, January 17, 2008; 32(2): 170 - 181.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
F. I. J. Crawford, C. L. Hodgkinson, E. Ivanova, L. B. Logunova, G. J. Evans, S. Steinlechner, and A. S. I. Loudon
Influence of torpor on cardiac expression of genes involved in the circadian clock and protein turnover in the Siberian hamster (Phodopus sungorus)
Physiol Genomics, November 14, 2007; 31(3): 521 - 530.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
N. J. Serkova, J. C. Rose, L. E. Epperson, H. V. Carey, and S. L. Martin
Quantitative analysis of liver metabolites in three stages of the circannual hibernation cycle in 13-lined ground squirrels by NMR
Physiol Genomics, September 11, 2007; 31(1): 15 - 24.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Yan, A. Burman, C. Nichols, L. Alila, L. C. Showe, M. K. Showe, B. B. Boyer, B. M. Barnes, and T. G. Marr
Detection of differential gene expression in brown adipose tissue of hibernating arctic ground squirrels with mouse microarrays
Physiol Genomics, April 13, 2006; 25(2): 346 - 353.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.