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Physiol. Genomics (July 30, 2002). doi:10.1152/physiolgenomics.00052.2002
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Articles in PresS, published online ahead of print July 30, 2002
Physiol Genomics, 10.1152/physiolgenomics.00052.2002
Submitted on May 3, 2002
Accepted on July 29, 2002

Microarray comparison of normal and W/Wv mice in the gastric fundus indicates a supersensitive phenotype

Gerard P Sergeant1, Roddy J Large1, Elizabeth A. H Beckett1, Catherine M McGeough1, Sean M Ward1, and Burton Horowitz1*

1 Departments of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, USA

* To whom correspondence should be addressed. E-mail: burt{at}physio.unr.edu.

Interstitial cells of Cajal (ICC) have been identified in specific areas throughout the smooth musculature of the gastro-intestinal (GI) tract. Located within the circular and longitudinal muscle layers of the gastric fundus lies a specific type of ICC, termed 'intramuscular' ICC or IC-IM. The principal function of this cell type is to act as 'mediators of excitatory and inhibitory enteric neurotransmission'. The functional role of these cells has been investigated using W/Wv mutant mice that specifically lack IC-IM and loss of enteric motor neurotransmission. The aim of the present study was to investigate differential gene expression in W/Wv mutant mice, from the tunica muscularis of the gastric fundus using a mouse cDNA microarray containing 1081 known genes. Verification of the microarray data was attained using real time 'quantitative' PCR (QPCR). Of the 1081 arrayed genes, 36 demonstrated differential expression by >2 fold in the W/Wv mice. An agreement rate of 50% (7 of 14 tested) was obtained using QPCR. Of the 7 confirmed changes in expression, several were indicative of a supersensitive phenotype, observed in denervation models. Expression of several putative neurotransmitter receptors including P2Y, the receptor for the inhibitory neurotransmitter ATP, was upregulated. The functional role of the P2Y receptor was also investigated using electrophysiological recordings. These results offer a new insight into the molecular changes that occur in W/Wv fundic smooth muscle and may also provide novel information with regard to the importance of IC-IM in enteric neurotransmission.




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