Physiol. Genomics Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiol. Genomics (April 10, 2007). doi:10.1152/physiolgenomics.00047.2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Table and Figures
Right arrow All Versions of this Article:
30/2/179    most recent
00047.2007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chalothorn, D.
Right arrow Articles by Faber, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chalothorn, D.
Right arrow Articles by Faber, J. E.
Submitted on March 1, 2007
Accepted on April 4, 2007

Collateral density, remodeling and VEGF-A expression differ widely between mouse strains

Dan Chalothorn1, Jason A Clayton1, Hua Zhang1, Daniel Pomp1, and James E. Faber1*

1 Cell and Molecular Physiology, University of North Carolina, Chapel Hill, North Carolina, United States

* To whom correspondence should be addressed. E-mail: james_faber{at}med.unc.edu.

Substantial variability exists in collateral density and ischemia-induced collateral growth among species. To begin to probe the underlying mechanisms, which are unknown, we characterized two mouse strains with marked differences in both parameters. Immediately after femoral artery ligation, collateral and foot perfusion were lower in BALB/c than C57BL/6 (P<0.05 here and below), suggesting fewer pre-existing collaterals. This was confirmed with angiography and immunohistochemistry (~35% fewer collaterals in the BALB/c's thigh). Recovery of hindlimb perfusion was attenuated in BALB/c, in association with 54% less collateral remodeling, reduced angiogenesis, greater ischemia and more impaired hindlimb use. Densities of CD45+ and CD4+ leukocytes around collaterals increased similarly, but TNF-{alpha} expression was 50% lower in BALB/c, which may contribute to reduced collateral remodeling. In normal tissues, compared to C57BL/6, BALB/c exhibit an altered arterial branching pattern, and like skeletal muscle above, have 30% fewer collaterals in intestine and, remarkably, almost none in pial circulation --resulting in greatly impaired perfusion after cerebral artery occlusion. Ischemic induction of VEGF-A was attenuated in BALB/c. Analysis of a C57BL/6-x-BALB/c recombinant inbred strain dataset identified a quantitative trait locus for VEGF-A mRNA abundance at or near the Vegfa locus that associates with lower expression in BALB/c. This suggests a cis-acting polymorphism in the Vegfa gene in BALB/c could contribute to reduced VEGF-A expression and, in turn, the above deficiencies in this strain. These findings suggest these strains offer a model to investigate genetic determinants of collateral formation and growth in ischemia.




This article has been cited by other articles:


Home page
 Circ. Res.Home page
W. M. Chilian and Y. F. Pung
Vascular Endothelial Growth Factor and the Collateral Circulation: The Story Continues
Circ. Res., October 24, 2008; 103(9): 905 - 906.
[Full Text] [PDF]

Home page
 Circ. Res.Home page
J. A. Clayton, D. Chalothorn, and J. E. Faber
Vascular Endothelial Growth Factor-A Specifies Formation of Native Collaterals and Regulates Collateral Growth in Ischemia
Circ. Res., October 24, 2008; 103(9): 1027 - 1036.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. Cheng, H. Zhang, X. Yang, E. Tzima, K. L. Ewalt, P. Schimmel, and J. E. Faber
Effect of mini-tyrosyl-tRNA synthetase on ischemic angiogenesis, leukocyte recruitment, and vascular permeability
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2008; 295(4): R1138 - R1146.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
T. Kinnaird, E. Stabile, S. Zbinden, M.-S. Burnett, and S. E. Epstein
Cardiovascular risk factors impair native collateral development and may impair efficacy of therapeutic interventions
Cardiovasc Res, May 1, 2008; 78(2): 257 - 264.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. S. Isenberg, D. D. Roberts, and W. A. Frazier
CD47: A New Target in Cardiovascular Therapy
Arterioscler. Thromb. Vasc. Biol., April 1, 2008; 28(4): 615 - 621.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
L. M. Messina
Elucidating the Genetic Basis of Peripheral Arterial Disease: Identification of a Quantitative Trait Locus That Determines the Phenotypic Response to Experimental Hindlimb Ischemia
Circulation, March 4, 2008; 117(9): 1127 - 1129.
[Full Text] [PDF]

Home page
 CirculationHome page
A. O. Dokun, S. Keum, S. Hazarika, Y. Li, G. M. Lamonte, F. Wheeler, D. A. Marchuk, and B. H. Annex
A Quantitative Trait Locus (LSq-1) on Mouse Chromosome 7 Is Linked to the Absence of Tissue Loss After Surgical Hindlimb Ischemia
Circulation, March 4, 2008; 117(9): 1207 - 1215.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
W. Schaper
Prevention of Tissue Death by Killer Cells?: The Role of the Immune System in Arteriogenesis
Arterioscler. Thromb. Vasc. Biol., November 1, 2007; 27(11): 2273 - 2274.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.