| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Cardiovascular Surgery, Hospital for Sick Children, Toronto, ON, Canada
2 Cardiology, Heart and Stroke Richard Lewar Centre of Excellence, Toronto, ON, Canada
3 Addenbrooke's Centre of Clinical Investigations, University of Cambridge, Canbridge, UK, United Kingdom
4 Cardiology, Hospital for Sick Children, Toronto, ON, Canada
5 Respirology, University of Toronto, Toronto, ON, Canada
* To whom correspondence should be addressed. E-mail: andrew.redington{at}sickkids.ca.
<Background: Remote ischemic preconditioning (IPC) reduces tissue injury caused by ischemia-reperfusion (IR) in distant organs. We tested the hypothesis that remote IPC modifies inflammatory gene transcription in humans. Methods and Results: Using a microarray method, we demonstrated that a simple model of brief forearm ischemia suppresses pro-inflammatory gene expression in circulating leukocytes. Genes encoding key proteins involved in cytokine synthesis, leukocyte chemotaxis, adhesion and migration, exocytosis, innate immunity signaling pathways and apoptosis were all suppressed within 15 minutes (early phase IPC) and more so after 24 hours (second window IPC). Changes in leukocyte CD11b expression measured by flow cytometry mirrored this pattern, there being a significant (p=0.01) reduction at 24 hours. Conclusion: The results of this study show that the remote IPC stimulus modifies leukocyte inflammatory gene expression. This effect may contribute to the protective effect of IPC against IR injury, and may have broader implications in other inflammatory processes.
This article has been cited by other articles:
|
|
 |
|
  D. J. Hausenloy and D. M. Yellon Remote ischaemic preconditioning: underlying mechanisms and clinical application Cardiovasc Res, August 1, 2008; 79(3): 377 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lucchinetti, J. Aguirre, J. Feng, M. Zhu, M. Suter, D. R. Spahn, L. Harter, and M. Zaugg Molecular Evidence of Late Preconditioning After Sevoflurane Inhalation in Healthy Volunteers Anesth. Analg., September 1, 2007; 105(3): 629 - 640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Arab, I. E. Konstantinov, C. Boscarino, E. Cukerman, A. Mori, J. Li, P. P. Liu, A. N. Redington, and J. G. Coles Early gene expression profiles during intraoperative myocardial ischemia-reperfusion in cardiac surgery J. Thorac. Cardiovasc. Surg., July 1, 2007; 134(1): 74 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J Hausenloy and D. M Yellon The evolving story of "conditioning" to protect against acute myocardial ischaemia-reperfusion injury Heart, June 1, 2007; 93(6): 649 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Schmidt, M. Smerup, I. E. Konstantinov, M. Shimizu, J. Li, M. Cheung, P. A. White, S. B. Kristiansen, K. Sorensen, V. Dzavik, et al. Intermittent peripheral tissue ischemia during coronary ischemia reduces myocardial infarction through a KATP-dependent mechanism: first demonstration of remote ischemic perconditioning Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1883 - H1890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R K Kharbanda, J Li, I E Konstantinov, M M H Cheung, P A White, H Frndova, J Stokoe, P Cox, M Vogel, G Van Arsdell, et al. Remote ischaemic preconditioning protects against cardiopulmonary bypass-induced tissue injury: a preclinical study Heart, October 1, 2006; 92(10): 1506 - 1511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M.H. Cheung, R. K. Kharbanda, I. E. Konstantinov, M. Shimizu, H. Frndova, J. Li, H. M. Holtby, P. N. Cox, J. F. Smallhorn, G. S. Van Arsdell, et al. Randomized Controlled Trial of the Effects of Remote Ischemic Preconditioning on Children Undergoing Cardiac Surgery: First Clinical Application in Humans J. Am. Coll. Cardiol., June 6, 2006; 47(11): 2277 - 2282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. E. Konstantinov and A. N. Redington Linking gene expression, nuclear factor kappa B, remote ischemic preconditioning, and transplantation: A quest for an elusive Holy Grail or a road to an amazing discovery? J. Thorac. Cardiovasc. Surg., February 1, 2006; 131(2): 507 - 509. [Full Text] [PDF]
|
|
|
|
|
|
I. E. Konstantinov, S. Arab, J. Li, J. G. Coles, C. Boscarino, A. Mori, E. Cukerman, F. Dawood, M. M.H. Cheung, M. Shimizu, et al. The remote ischemic preconditioning stimulus modifies gene expression in mouse myocardium J. Thorac. Cardiovasc. Surg., November 1, 2005; 130(5): 1326 - 1332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Seeburger, J. Hoffmann, H. P. Wendel, G. Ziemer, and H. Aebert Gene Expression Changes in Leukocytes During Cardiopulmonary Bypass Are Dependent on Circuit Coating Circulation, August 30, 2005; 112(9_suppl): I-224 - I-228. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
