Physiol. Genomics AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiol. Genomics (July 2, 2002). doi:10.1152/physiolgenomics.00044.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
10/2/79    most recent
00044.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petriv, O. I
Right arrow Articles by Titorenko, V. I
Right arrow Search for Related Content
PubMed
Right arrow Articles by Petriv, O. I
Right arrow Articles by Titorenko, V. I

Articles in PresS, published online ahead of print June 27, 2002
Physiol Genomics, 10.1152/physiolgenomics.00044.2002
Submitted on April 16, 2002
Accepted on June 18, 2002

RNA interference of peroxisome-related genes in C. elegans - a new model for the human peroxisomal disorders

Oleh I Petriv1, David B Pilgrim2, Richard A Rachubinski1*, and Vladimir I Titorenko3

1 Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada
2 Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
3 Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada; Department of Biology, Concordia University, Montreal, Quebec, Canada

* To whom correspondence should be addressed. E-mail: rick.rachubinski{at}ualberta.ca.

RNA-mediated interference (RNAi) for the post-transcriptional silencing of genes was used to evaluate the importance of various peroxisomal enzymes and peroxins for the development of Caenorhabditis elegans and to compare the roles of these proteins in the nematode to their roles in yeasts and humans. The nematode counterparts of the human ATP-binding cassette half-transporters, the enzymes alkyldihydroxyacetonephosphate synthase and {Delta}3,5-{Delta}2,4-dienoyl-CoA isomerase, the receptors for peroxisomal membrane and matrix proteins (Pex19p and Pex5p), and components of the docking and translocation machineries for matrix proteins (Pex13p and Pex12p) are essential for the development of C. elegans. Unexpectedly, RNAi silencing of the acyl-CoA synthetase-mediated activation of fatty acids, the {alpha}- and ß-oxidation of fatty acids, the intraperoxisomal decomposition of hydrogen peroxide, and the peroxins Pex1p, Pex2p and Pex6p had no apparent effect on C. elegans development. The described analysis of functional gene knockouts through RNAi provides a basis for the use of C. elegans as a valuable model system with which to study the molecular and physiological defects underlying the human peroxisomal disorders.




This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
A. Boll and M. Schrader
Elongation of Peroxisomes as an Indicator for Efficient Dynamin-like Protein 1 Knock Down in Mammalian Cells
J. Histochem. Cytochem., August 1, 2005; 53(8): 1037 - 1040.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. I. Petriv and R. A. Rachubinski
Lack of Peroxisomal Catalase Causes a Progeric Phenotype in Caenorhabditis elegans
J. Biol. Chem., May 7, 2004; 279(19): 19996 - 20001.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
V. I. Titorenko and R. A. Rachubinski
The peroxisome: orchestrating important developmental decisions from inside the cell
J. Cell Biol., March 1, 2004; 164(5): 641 - 645.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Thieringer, B. Moellers, G. Dodt, W.-H. Kunau, and M. Driscoll
Modeling human peroxisome biogenesis disorders in the nematode Caenorhabditis elegans
J. Cell Sci., May 1, 2003; 116(9): 1797 - 1804.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.