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Articles in PresS, published online ahead of print August 8, 2001
Physiol Genomics, 10.1152/physiolgenomics.00044.2001
Submitted on June 4, 2001
Accepted on August 2, 2001
1 Physiology, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK, USA
2 Nephrology Section, Tulane University Medical Center, New Orleans, LA, USA
3 Pediatrics, State University of New York (SUNY), Brooklyn, NY, USA
* To whom correspondence should be addressed. E-mail: ricardo-saban{at}ouhsc.edu.
Mast cell numbers are significantly increased in bladder disorders including malignancy and interstitial cystitis, but their precise role has been difficult to determine. We characterized the role of mast cell on gene regulation associated with antigen-induced bladder inflammation in mice. For this purpose, we examined the responses in mast cell-deficient (KitW/KitW-v), congenic normal (+/+), and KitW/KitW-v mice that were reconstituted with bone marrow stem cells (BMR) to restore mast cells. All mice were actively sensitized and challenged intravesically with either saline or specific antigen. Bladder inflammation occurred in +/+ and BMR, but not the KitW/KitW-v mice. Gene expression was determined using mouse cDNA expression arrays. Self-organizing maps, performed without preconditions, indicated gene expression changes dependent on the presence of mast cells. These genes were up-regulated in bladders isolated from antigen-challenge of +/+; not significantly altered in KitW/KitW-v, and were up-regulated in BMR mice. Taken together these results demonstrate an important role for mast cells in allergic cystitis and indicate that mast cells can alter their environment by regulating tissue gene-expression.
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