| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print May 15, 2002
Physiol Genomics, 10.1152/physiolgenomics.00035.2002
Submitted on March 28, 2002
Accepted on May 8, 2002
1 Cardiovascular Research, Genentech, South San Francisco, CA, USA
2 Molecular Biology, Genentech, South San Francisco, CA, USA
3 Pathology, Genentech, South San Francisco, CA, USA
4 Bioinformatics, Genentech, South San Francisco, CA, USA
* To whom correspondence should be addressed. E-mail: meg570{at}attbi.com.
The objective of this study was to use gene expression data from well-defined cell culture models, in combination with expression data from diagnostic samples of human diseased tissues, to identify potential therapeutic targets and markers of disease. Using Affymetrix oligonucleotide array technology, we identified a common profile of genes upregulated during endothelial morphogenesis into tube-like structures in three in vitro models of angiogenesis. Rigorous data selection criteria were used to identify a list of over 1000 genes whose expression was increased more than two-fold over base-line at either 4, 8, 24, 40 or 50 hours. To further refine and prioritize this list, we used standard bioinformatic algorithms to identify potential transmembrane and secreted proteins. We then overlapped this gene set with genes upregulated in colon tumors versus normal colon, resulting in a subset of 128 genes in common with our endothelial list. We removed from this list those genes expressed in 6 different colon tumor lines, resulting in a list of 24 putative, vascular-specific angiogenesis-associated genes. Three genes, gp34, stanniocalcin 1, GA733-1 were expressed at levels 10 fold or more in colon tumors compared to normal mucosa. We validated the vascular specific expression of one of these genes, STC-1, by in situ hybridization. The ability to combine in vitro and in vivo data sets should permit one to identify putative angiogenesis target genes in various tumors, chronic inflammation, and other disorders where therapeutic manipulation of angiogenesis is a desirable treatment modality.
This article has been cited by other articles:
|
|
 |
|
  E. A. Ashley, J. M. Spin, R. Tabibiazar, and T. Quertermous Frontiers in Nephrology: Genomic Approaches to Understanding the Molecular Basis of Atherosclerosis J. Am. Soc. Nephrol., November 1, 2007; 18(11): 2853 - 2862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Y. Yeung, K. P. Lai, H. Y. Chan, N. K. Mak, G. F. Wagner, and C. K. C. Wong Hypoxia-Inducible Factor-1-Mediated Activation of Stanniocalcin-1 in Human Cancer Cells Endocrinology, November 1, 2005; 146(11): 4951 - 4960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J.M. Best, J. W. Gillespie, Y. Yi, G. V.R. Chandramouli, M. A. Perlmutter, Y. Gathright, H. S. Erickson, L. Georgevich, M. A. Tangrea, P. H. Duray, et al. Molecular Alterations in Primary Prostate Cancer after Androgen Ablation Therapy Clin. Cancer Res., October 1, 2005; 11(19): 6823 - 6834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Song, J. L. Barth, Y. Yu, K. Lu, A. Dashti, Y. Huang, C. K. Gittinger, W. S. Argraves, and T. J. Lyons Effects of Oxidized and Glycated LDL on Gene Expression in Human Retinal Capillary Pericytes Invest. Ophthalmol. Vis. Sci., August 1, 2005; 46(8): 2974 - 2982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Paciga, E. R. Hirvi, K. James, and G. F. Wagner Characterization of big stanniocalcin variants in mammalian adipocytes and adrenocortical cells Am J Physiol Endocrinol Metab, August 1, 2005; 289(2): E197 - E205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. N. Hahn, Z. J. Su, C. J. Drogemuller, A. Tsykin, S. R. Waterman, P. J. Brautigan, S. Yu, G. Kremmidiotis, A. Gardner, P. J. Solomon, et al. Expression profiling reveals functionally important genes and coordinately regulated signaling pathway genes during in vitro angiogenesis Physiol Genomics, June 16, 2005; 22(1): 57 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Finley, N. Arora, B. Zhu, L. Gallagher, and T. J. Fahey III Molecular Profiling Distinguishes Papillary Carcinoma from Benign Thyroid Nodules J. Clin. Endocrinol. Metab., July 1, 2004; 89(7): 3214 - 3223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Unthank, K. M. Sheridan, and M. C. Dalsing Collateral Growth in the Peripheral Circulation: A Review Vascular and Endovascular Surgery, July 1, 2004; 38(4): 291 - 313. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Liu, H. Jia, D. I. R. Holmes, A. Stannard, and I. Zachary Vascular Endothelial Growth Factor-Regulated Gene Expression in Endothelial Cells: KDR-Mediated Induction of Egr3 and the Related Nuclear Receptors Nur77, Nurr1, and Nor1 Arterioscler. Thromb. Vasc. Biol., November 1, 2003; 23(11): 2002 - 2007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mirotsou, C. M.H. Watanabe, P. G. Schultz, R. E. Pratt, and V. J. Dzau Elucidating the molecular mechanism of cardiac remodeling using a comparative genomic approach Physiol Genomics, October 17, 2003; 15(2): 115 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ho, E. Yang, G. Matcuk, D. Deng, N. Sampas, A. Tsalenko, R. Tabibiazar, Y. Zhang, M. Chen, S. Talbi, et al. Identification of endothelial cell genes by combined database mining and microarray analysis Physiol Genomics, May 13, 2003; 13(3): 249 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. C. Aird, S. B. Glueck, V. J. Dzau, and R. E. Pratt Separating the wheat from the chaff: Focus on "In silico data filtering to identify new angiogenesis targets from a large in vitro gene profile data set" Physiol Genomics, July 12, 2002; 10(1): 1 - 3. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
