| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print July 9, 2002
Physiol Genomics, 10.1152/physiolgenomics.00030.2002
Submitted on March 21, 2002
Accepted on July 8, 2002
1 Division of Cardiology, University of Cincinnati Medical Center, Cincinnati, Ohio, USA
* To whom correspondence should be addressed. E-mail: dorngw{at}ucmail.uc.edu.
The frequency of single nucleotide polymorphisms in downstream signaling proteins was determined by combination heteroduplex high performance liquid chromatography and double stranded sequencing of genomic DNA from 96 to 144 congestive heart failure (CHF) patients. Analysis of fifty-six coding exons in nine signaling genes revealed seventeen novel and eight previously reported synonymous (no change in amino acid) SNPs, as well as one novel non-synonymous SNP in the Rad small G-protein. Because this initial analysis failed to detect numerous SNPs reported in the NCBI and Celera databases, double strand sequencing of relevant exons from 74 to 91 CHF patients was used to confirm the absence of ten previously reported non-synonymous SNPs. Our results show that synonymous SNPs are frequent in signaling protein genes, whereas non-synonymous SNPs are rare, suggesting a high degree of evolutionary conservation among these downstream signaling molecules. Comparisons of our results to the NCBI and Celera databases indicates that 56% of their SNP entries are not detected in our cohort. Importantly, while 31% of database SNPs were verified, 69% of SNPs detected in our cohort are not included in these databases. These findings indicate that caution may be warranted in relying exclusively on SNP databases as catalogs for polymorphic signaling protein genes.
This article has been cited by other articles:
|
|
 |
|
  S. B. Liggett, R. J. Kelly, R. R. Parekh, S. J. Matkovich, B. J. Benner, H. S. Hahn, F. M. Syed, A. S. Galvez, K. L. Case, N. McGuire, et al. A functional polymorphism of the G{alpha}q (GNAQ) gene is associated with accelerated mortality in African-American heart failure Hum. Mol. Genet., November 15, 2007; 16(22): 2740 - 2750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Hawke, S. B. Kanatous, C. M. Martin, S. C. Goetsch, and D. J. Garry Rad is temporally regulated within myogenic progenitor cells during skeletal muscle regeneration Am J Physiol Cell Physiol, February 1, 2006; 290(2): C379 - C387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Tantisira, K. M. Small, A. A. Litonjua, S. T. Weiss, and S. B. Liggett Molecular properties and pharmacogenetics of a polymorphism of adenylyl cyclase type 9 in asthma: interaction between {beta}-agonist and corticosteroid pathways Hum. Mol. Genet., June 15, 2005; 14(12): 1671 - 1677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Berman, Y. Wang, Z. Liu, Q. Dong, L.-A. Burke, L. A. Liotta, R. Fisher, and X. Wu A Functional Polymorphism in RGS6 Modulates the Risk of Bladder Cancer Cancer Res., September 15, 2004; 64(18): 6820 - 6826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Finlin, S. M. Crump, J. Satin, and D. A. Andres Regulation of voltage-gated calcium channel activity by the Rem and Rad GTPases PNAS, November 25, 2003; 100(24): 14469 - 14474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. N. Nikiforova, R. A. Lynch, P. W. Biddinger, E. K. Alexander, G. W. Dorn II, G. Tallini, T. G. Kroll, and Y. E. Nikiforov RAS Point Mutations and PAX8-PPAR{gamma} Rearrangement in Thyroid Tumors: Evidence for Distinct Molecular Pathways in Thyroid Follicular Carcinoma J. Clin. Endocrinol. Metab., May 1, 2003; 88(5): 2318 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Stephan and S. B. Glueck Containing multitudes: Focus on "Novel and nondetected human signaling protein polymorphisms" Physiol Genomics, September 3, 2002; 10(3): 127 - 129. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
