Submitted on February 17, 2006
Accepted on December 6, 2006
Congenic Strains Confirm Aerobic Running Capacity Quantitative Trait Loci on Rat Chromosome 16 and Identify Possible Intermediate Phenotypes
Justin A. Ways1, Brian M Smith2, John Christopher Barbato3, Ramona S Ramdath4, Krista M Pettee4, Sarah J DeRaedt4, David C Allison2, Lauren Gerard Koch5, Soon Jin Lee6, and George T. Cicila7*
1 Department of Physiology, Pharmacology, Metabolism, and Cardiovascular, University of Toledo College of Medicine, Ann Arbor, Ohio, United States
2 Surgery, University of Toledo College of Medicine, Toledo, Ohio, United States
3 Department of Cell Biology/ NC10, Cleveland Clinic Foundation, Cleveland, Ohio, United States
4 Physiology, Pharmacology, Metabolism, and Cardiovascular Sciences, University of Toledo College of Medicine, Toledo, Ohio, United States
5 Physical Medicine and Rehabilitation, University of Michigan, Ann Arbor, Michigan, United States
6 Physiology, Pharmacology & Molecular Medicine, Medical University of Ohio, Toledo, Ohio, United States
7 Physiology, Pharmacology, metabolism and Cardiovascular Sciences, Medical College of Ohio, Toledo, Ohio, United States
* To whom correspondence should be addressed. E-mail: george.cicila{at}utoledo.edu.
We previously identified two inbred rat strains divergent for treadmill aerobic running capacity (ARC), the low performing Copenhagen (COP) and the high performing DA rats, and used an F2(COPxDA) population to identify ARC quantitative trait loci (QTLs) on rat chromosome 16 (RNO16) and the proximal portion of rat chromosome 3 (RNO3). Two congenic rat strains were bred to further investigate these ARC QTLs by introgressing RNO16 and the proximal portion of RNO3 from DA rats into the genetic background of COP rats and were named COP.DA(chr 16) and COP.DA(chr 3), respectively. COP.DA(chr 16) rats had significantly greater ARC compared to COP rats (696.7±38.2 m vs. 571.9±27.5 m, P=0.03). COP.DA(chr 3) rats had increased, although not significant, ARC compared to COP rats (643.6±40.9 m vs. 571.9±27.5 m). COP.DA(chr 16) rats had significantly greater subcutaneous abdominal fat, as well as decreased fasting triglyceride levels, compared to COP rats (P<0.05), indicating that genes responsible for strain differences in fat metabolism are also located on RNO16. While this colocalization of QTLs may be coincidental, it is also possible that these differences in energy balance may be associated with the superior running performance of COP.DA(chr 16) consomic rats.
