| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Medicine, Cardiovascular Division, University of Minnesota, Minneapolis, MN, USA
* To whom correspondence should be addressed. E-mail: bache001{at}tc.umn.edu.
Chronic unloading of the failing heart with a left ventricular assist device (LVAD) can decrease cardiac mass and myocyte size and has the potential to improve contractile function. To study the effect of chronic ventricular unloading on myocardial gene expression, a microarray (U133A, Affymetrix) profiling gene expression was compared before and after LVAD support in 7 patients with idiopathic dilated cardiomyopathy and end-stage heart failure. On average, 1374±155 genes were reported as "increased" and 1629±45 as "decreased" after LVAD support. A total of 130 gene transcripts achieved the strict criteria for up-regulation and 49 gene transcripts for down-regulation after LVAD support. Up-regulated genes included a large proportion of transcription factors, genes related to cell growth/apoptosis/DNA repair, cell structure proteins, metabolism, and cell signaling/communication. LVAD support resulted in down-regulation of genes for a group of cytokines. To validate the array data, ten altered genes were confirmed by real-time RT-PCR. Further study showed that the phosphoinositide-3-kinase-forkhead protein pathway and proteins related to nitric oxide synthesis, including eNOS and dimethylarginine dimethylaminohydrolase isoform-1 (DDAH1, an enzyme regulating endogenous nitric oxide synthase activity), were significantly increased during the cardiac remodeling process. Increased eNOS and DDAH1 expression after LVAD support may contribute to improved endothelial function of the failing hearts.
This article has been cited by other articles:
|
|
 |
|
  G. W. Dorn II and S. J. Matkovich Put Your Chips on Transcriptomics Circulation, July 15, 2008; 118(3): 216 - 218. [Full Text] [PDF]
|
|
|
|
 |
|
 P. Philip-Couderc, N. I. Tavares, A. Roatti, R. Lerch, C. Montessuit, and A. J. Baertschi Forkhead Transcription Factors Coordinate Expression of Myocardial KATP Channel Subunits and Energy Metabolism Circ. Res., February 1, 2008; 102(2): e20 - e35. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Cappola Molecular Remodeling in Human Heart Failure J. Am. Coll. Cardiol., January 15, 2008; 51(2): 137 - 138. [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hall, E. J. Birks, S. Grindle, M. E. Cullen, P. J. Barton, J. E. Rider, S. Lee, S. Harwalker, A. Mariash, N. Adhikari, et al. Molecular signature of recovery following combination left ventricular assist device (LVAD) support and pharmacologic therapy Eur. Heart J., March 1, 2007; 28(5): 613 - 627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schiekofer, I. Shiojima, K. Sato, G. Galasso, Y. Oshima, and K. Walsh Microarray analysis of Akt1 activation in transgenic mouse hearts reveals transcript expression profiles associated with compensatory hypertrophy and failure Physiol Genomics, October 11, 2006; 27(2): 156 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Donahue, D. A. Marchuk, and H. A. Rockman Redefining Heart Failure: The Utility of Genomics J. Am. Coll. Cardiol., October 3, 2006; 48(7): 1289 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hannenhalli, M. E. Putt, J. M. Gilmore, J. Wang, M. S. Parmacek, J. A. Epstein, E. E. Morrisey, K. B. Margulies, and T. P. Cappola Transcriptional Genomics Associates FOX Transcription Factors With Human Heart Failure Circulation, September 19, 2006; 114(12): 1269 - 1276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Heerdt, S. Klotz, and D. Burkhoff Cardiomyopathic Etiology and SERCA2a Reverse Remodeling During Mechanical Support of the Failing Human Heart Anesth. Analg., January 1, 2006; 102(1): 32 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, Y. Li, P. Zhang, J. H. Traverse, M. Hou, X. Xu, M. Kimoto, and R. J. Bache Dimethylarginine dimethylaminohydrolase and endothelial dysfunction in failing hearts Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2212 - H2219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liang and B. Ventura Physiological genomics in PG and beyond: July to September 2005 Physiol Genomics, October 17, 2005; 23(2): 119 - 124. [Full Text] [PDF]
|
|
|
|
|
|
E. J. Birks, J. L. Hall, P. J.R. Barton, S. Grindle, N. Latif, J. P. Hardy, J. E. Rider, N. R. Banner, A. Khaghani, L. W. Miller, et al. Gene Profiling Changes in Cytoskeletal Proteins During Clinical Recovery After Left Ventricular-Assist Device Support Circulation, August 30, 2005; 112(9_suppl): I-57 - I-64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Kittleson, K. M. Minhas, R. A. Irizarry, S. Q. Ye, G. Edness, E. Breton, J. V. Conte, G. Tomaselli, J. G. N. Garcia, and J. M. Hare Gene expression analysis of ischemic and nonischemic cardiomyopathy: shared and distinct genes in the development of heart failure Physiol Genomics, May 11, 2005; 21(3): 299 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Margulies, S. Matiwala, C. Cornejo, H. Olsen, W. A. Craven, and D. Bednarik Mixed Messages: Transcription Patterns in Failing and Recovering Human Myocardium Circ. Res., March 18, 2005; 96(5): 592 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Iacobas, S. Iacobas, W. E. I. Li, G. Zoidl, R. Dermietzel, and D. C. Spray Genes controlling multiple functional pathways are transcriptionally regulated in connexin43 null mouse heart Physiol Genomics, February 10, 2005; 20(3): 211 - 223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Kittleson, S. Q. Ye, R. A. Irizarry, K. M. Minhas, G. Edness, J. V. Conte, G. Parmigiani, L. W. Miller, Y. Chen, J. L. Hall, et al. Identification of a Gene Expression Profile That Differentiates Between Ischemic and Nonischemic Cardiomyopathy Circulation, November 30, 2004; 110(22): 3444 - 3451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Huebert, Q. Li, N. Adhikari, N. J. Charles, X. Han, M.-K. Ezzat, S. Grindle, S. Park, S. Ormaza, D. Fermin, et al. Identification and regulation of Sprouty1, a negative inhibitor of the ERK cascade, in the human heart Physiol Genomics, August 11, 2004; 18(3): 284 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Hall, S. Grindle, X. Han, D. Fermin, S. Park, Y. Chen, R. J. Bache, A. Mariash, Z. Guan, S. Ormaza, et al. Genomic profiling of the human heart before and after mechanical support with a ventricular assist device reveals alterations in vascular signaling networks Physiol Genomics, May 19, 2004; 17(3): 283 - 291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Razeghi and H. Taegtmeyer Activity of the Akt/GSK-3{beta} pathway in the failing human heart before and after left ventricular assist device support Cardiovasc Res, January 1, 2004; 61(1): 196 - 197. [Full Text] [PDF]
|
|
|
|
|
|
H. A Baba and B. Levkau Experimental and clinical parameters of cardiac reverse remodeling after LVAD support: a call for uniformity Cardiovasc Res, January 1, 2004; 61(1): 198 - 199. [Full Text] [PDF]
|
|
|
|
|
|
K. B. Margulies Blocking Stretch-Induced Myocardial Remodeling Circ. Res., November 28, 2003; 93(11): 1020 - 1022. [Full Text] [PDF]
|
|
|
|
|
|
M. M. Chen, E. A. Ashley, D. X.F. Deng, A. Tsalenko, A. Deng, R. Tabibiazar, A. Ben-Dor, B. Fenster, E. Yang, J. Y. King, et al. Novel Role for the Potent Endogenous Inotrope Apelin in Human Cardiac Dysfunction Circulation, September 23, 2003; 108(12): 1432 - 1439. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
