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2 induce adipogenesis of NIH3T3 cells with similar efficiency and kinetics
1 Department of Molecular Pharmacology, AstraZeneca R&D Molndal, Molndal, Sweden
2 Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund, Sweden
3 Department of Molecular Sciences, AstraZeneca R&D Molndal, Molndal, Sweden
4 Department of Mathematical Statistics, Chalmers Technical University, Gotheborg, Sweden
* To whom correspondence should be addressed. E-mail: Peter.Akerblad{at}astrazeneca.se.
Differentiation of multipotent mesenchymal stem cells into lipid accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used AffymetrixTM micro-arrays to compare the adipogenic differentiation pathways of NIH3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of EBF-1 or PPAR
2. These experiments revealed that commitment to the adipogenic pathway in the NIH3T3 cells was not reflected in gene expression patterns until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR2 induced different sets of genes while the similarities increased upon differentiation and that several genes linked to adipocyte differentiation was transiently induced also in the vector transduced cells. These data suggests that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR2 induce adipocyte differentiation with comparable kinetics and efficiency.
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