Animals pooled for Affymetrix Experiments
Since injured spinal cord segments yield lesser
quantities of RNA, and since 10 mg
of RNA was required for each Affymetrix target preparation, animals were pooled
without regard to sex. The D1 segment had less cell loss due to injury and
therefore provided sufficient RNA to preclude pooling. The table below lists specific animal numbers and
sexes pooled.
|
I Segment |
| Sample |
Hrs Post
Injury |
Group* |
Number of
animals |
Animal codes |
Sexes |
| 0 |
0 |
A |
3 |
66-68 |
M, M, M |
| 0A |
0 |
B |
2 |
92, 94 |
F, M |
| 0B |
0 |
C |
2 |
93, 95 |
F, M |
| 6A |
6 |
A |
2 |
97, 99 |
F, M |
| 6B |
6 |
B |
1 |
98 |
F |
| 6C |
6 |
-- |
3 |
75-77 |
F, F, F |
| 6D |
6 |
C |
1 |
107 |
M |
| 12 |
12 |
A |
3 |
84-86 |
M, M, M |
| 12B |
12 |
B |
2 |
104, 105 |
F, F |
| 12C |
12 |
C |
1 |
106 |
F |
| 24 |
24 |
A |
3 |
69-71 |
M, M, M |
| 24A |
24 |
B |
2 |
100, 103 |
M, F |
| 24B |
24 |
C |
2 |
101, 102 |
M, F |
| 48 |
48 |
A |
3 |
72-74 |
M, M, M |
| 48B |
48 |
B |
2 |
HT70, HT71 |
M, M |
| 48C |
48 |
C |
2 |
HT58, HT59 |
M, M |
| D1 Segment |
| Sample |
Hrs Post
Injury |
Group* |
Number of
animals |
Animal codes |
Sexes |
| 0A |
0 |
A |
1 |
92 |
F |
| 0C |
0 |
B |
1 |
94 |
M |
| 0D |
0 |
C |
1 |
95 |
M |
| 6A |
6 |
A |
1 |
96 |
M |
| 6B |
6 |
B |
1 |
98 |
F |
| 6C |
6 |
C |
1 |
99 |
F |
| 12A |
12 |
A |
1 |
104 |
F |
| 12B |
12 |
B |
1 |
105 |
F |
| 12C |
12 |
C |
1 |
106 |
F |
| 24A |
24 |
A |
1 |
101 |
M |
| 24B |
24 |
B |
1 |
102 |
F |
| 24C |
24 |
C |
1 |
103 |
F |
| 48A |
48 |
A |
1 |
HT 70 |
M |
| 48B |
48 |
B |
1 |
HT 71 |
M |
| 48C |
48 |
C |
1 |
HT 58 |
M |
*Group letter matches downloadable data table.
|
