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| gene product | age effect in mice (fold change) | ORF mouse |
UniGene Hs... | SAGE catg... | # tags young |
# tags old |
Microarray result (human) | Microarray fold change (human) | Decision12 |
| Mitochondrial Sarcomeric Creatine Kinase | +3.8 | AA106112 | 80691 | tggaatgagc | 24 | 25 | D | -1.9 | Dis SA |
| Synaptic Vesicle Protein 2 | +3.8 | AA0717771 | 8071 | aagtgaaaaa gcattcagtt ttctacccca taacaagtaa |
0 0 0 0 |
0 0 0 0 |
a) NC b) absent both |
a) +1.2 | ND |
| Ypt 1/ras-related GTP Binding Protein | +3.6 | Y00094 | 3642 | cataaagttt | 1 | 0 | NC | +1.1 | Dis A |
| Methyl CpG Binding Protein | +3.5 | W10855 | 3239 | ccaaggccaa | 1 | 0 | NC | +1.1 | Dis A |
| Heat Shock 27 kDa Protein | +3.5 | W08057 | 76067 | cccaagctag | 65 | 66 | NC | +1.1 | Dis SA |
| Serum Amyloid A Isoform 4 | +3.5 | M17790 | 181062 | gtgcggagga | 0 | 1 | NC, absent old |
-1.3 | Dis A |
| GDF-9 | +3.5 | L06444 | 248113 | tgtacctgcc | 0 | 0 | no probes | ND | |
| Heat Shock 71 kDa Protein | +3.4 | AA114576 | 1804142 | ccaggaggaa | 42 | 28 | NC | -1.2 | Dis SA |
| Transcription Regulatory Protein SWI3 | +3.3 | W84988 | 236030 | cattttatat catttttata |
0 0 |
0 0 |
absent both | ND | |
| U2AF | +3.2 | X64587 | 7655 | cagggcctga | 0 | 0 | absent both | ND | |
| ARF5 | +3.2 | D87902 | 77541 | ttctctctgt | 2 | 2 | NC, absent yng |
+1.2 | ND |
| LRG-21 | +3.0 | U19118 | 460 | tagctgtttt | 0 | 0 | no probes | ND | |
| RabB | +2.9 | AA068057 | 184627 | tagaaaagac taatgcagat |
1 2 |
0 0 |
NC | +1.1 | Dis SA |
| Beta-Hexosaminidase | +2.9 | U05837 | 119403 | agcacattct gattacctgt | 0 0 |
0 0 |
absent both | ND | |
| Protein Kinase C Inhibitor 1 Homolog | +2.8 | W85446 | 256697 | gcttatagtc gctaaaaaaa gcttataaaa |
3 1 0 |
1 1 0 |
no probes | Dis S | |
| Pre-B Cell Enhancing Factor Precursor | +2.7 | AA060167 | 239138 | gccttaacaa gaaataaagt |
2 0 |
0 0 |
NC | -1.4 | Dis SA |
| Serine-2 Ultrahigh Sulfur Protein | +2.7 | M37760 | 2743 | ccaagcaaga | 0 | 0 | absent both | ND | |
| G25K GTP-Binding Protein | +2.7 | AA096992 | 278736 | cttgtggatg acaaacctta | 0 0 |
0 0 |
no probes | ND | |
| Adaptin Complex Small Chain Homolog | +2.7 | AA008255 | 154782 | agagcagaag agagcagaaa agagcaaaaa |
0 1 2 |
0 0 1 |
NC | +1.4 | Dis S |
| EIF-4A-II | +2.6 | AA1665021 | 173912 | tcttaatgaa | 5 | 10 | no probes | Con S | |
| POU-domain protein | +2.6 | X66602 | 182505 | ctgctgtgtt | 0 | 1 | absent both | ND | |
| NK 10 | +2.6 | X79828 | 192662 | taggaaagaa | 0 | 0 | no probes | ND | |
| GADD45 | +2.6 | L28177 | 80409 | aaaagttatt | 0 | 0 | no probes | ND | |
| Nucleotide Pyrophosphatase | +2.5 | W509411 | 11951 | ttatgtgact | 0 | 0 | no probes | ND | |
| Neurotrophin-3 | +2.5 | X53257 | 99171 | tagcatataa | 0 | 0 | NC | -2.5 | Dis A |
| Aldehyde Dehydrogenase II | +2.4 | M74570 | 76392 | aatatacaga tctgcttgtc | 0 0 |
0 0 |
D | -1.6 | Dis A |
| Sox17 | +2.4 | D49473 | 97984 | caggaccacc | 0 | 0 | no probes | ND | |
| Zinc Finger Protein 43 (HTF6) | +2.3 | AA117284 | 74107 | gttgtcacac | 0 | 0 | NC | +1.1 | Dis A |
| Beta-centractin | +2.3 | W63835 | 2477 | tcaggctgtt | 3 | 1 | NC | +1.2 | Dis S |
| Phosphatidylcholine-transfer Protein | +2.2 | AA089097 | 283722 | tcattgtaat | 4 | 5 | no probes | ND | |
| Protease Do Precursor | +2.2 | AA059662 | 75111 | tttccctcaa | 0 | 0 | no probes | ND | |
| HIC-5 | +2.2 | L22482 | 25511 | ctggggctgc caccttctgc | 0 0 |
0 0 |
absent both | ND | |
| AP-2 Beta | +2.2 | X78197 | 33102 | gacaagatgt | 0 | 0 | absent both | ND | |
| IGF Binding Protein | +2.2 | AA059664 | 102171 | ctaacggggc | 0 | 0 | NC | -1.3 | Dis A |
| Alpha Globin | +2.2 | V00714 | 2725724 2515774 |
cccaacgcgc cttcttgccc |
38 28 |
87 39 |
no probes | Con S | |
| rhoB | +2.2 | X99963 | 204354 | gccgtgcgca gcggccatcc cacacagttt | 0 0 0 |
0 0 0 |
no probes | ND | |
| Dynactin | +2.2 | AA014024 | 74617 | tgcggctggt | 2 | 3 | absent both | ND | |
| TNZ2 | +2.2 | X65627 | 76053 | gccttccaat | 5 | 4 | NC | +1.3 | ND |
| GTP-Binding Protein (IRG-47) | +2.1 | X95503 | 75825 | gatccataat cattcagata atcataatgt gcttacgatt |
0 0 0 0 |
0 0 0 0 |
NC | -1.1 | Dis A |
| FBJ-MuSV | +2.1 | V00727 | 25647 | tggaaagtga | 8 | 7 | no probes | ND | |
| pp2.5 | +2.1 | X12807 | 101799 | aagagtgctt | 1 | 0 | no probes | ND | |
| MAGP | +2.1 | W08049 | 58882 | cagttgtcta5 | 0 | 0 | NC | 1 | Dis A |
| CO-029 | +2.1 | AA066425 | 84072 | ccaaagctat | 0 | 1 | I | +2.1 | Con A |
| POLYA+ RNA Export Protein | +2.1 | W82998 | 40323 | cttattcctt | 0 | 1 | a)NC b)NC |
a)-1.1 b)+1.2 |
Dis A |
| mTGIF | +2.1 | X89749 | 90077 | tggaacagga | 0 | 1 | NC | 1 | Dis A |
| GDP-Dissociation Inhibitor | +2.1 | L07918 | 83656 | ctggcccaag ctggcccgag ctggcgcgag |
0 0 0 |
1 0 0 |
no probes | ND | |
| PEA3 | +2.1 | X63190 | 77711 | cagaaggtgg | 0 | 2 | no probes | Con S | |
| Dihydropyridine-sensitive L-type Ca channel | +2.0 | AA061886 | 147989 | gatgctgagc | 6 | 11 | NC | +1.5 | Con SA |
| Rac 1 | +1.8 | X57277 | 286250 | gctaaggaga | 1 | 3 | no probes | Con S | |
| Proteasome Z subunit | -1.7 | D83585 | 118065 | tggctagtgt | 9 | 7 | NC | -1.2 | ND |
| Glycerophosphate dehydrogenase | -1.9 | M13366 | 25478 | ccaccacatt | 2 | 4 | NC | -1.2 | Dis S |
| Glucose-6-phosphate isomerase | -1.9 | AA071776 | 944 | tagaaaaata | 9 | 4 | D | -1.6 | Con SA |
| NADP transhydrogenase | -2.0 | Z49204 | 18136 | gtgatgtaca ttggcaaaaa |
0 1 |
0 0 |
D | -1.6 | Con A |
| IPP-2 | -2.0 | AA041826 | 267819 | acttaagtac | 1 | 1 | no probes | ND | |
| ATP synthase A chain | -2.0 | AA106406 | mt gene6 | cacctaattg | 1304 | 936 | no probes | Con S | |
| PMP35 | -2.0 | L27842 | 180612 | tgattctaaa | 3 | 0 | NC | -1.5 | Con SA |
| Zinc finger protein 32 | -2.0 | AA044497 | 78765 | gcaaaatcca | 1 | 0 | NC | -1.3 | ND |
| Signal recognition particle receptor | -2.0 | W53731 | 75730 | ttcccttcct ttccccttcc |
0 3 |
0 2 |
absent both | ND | |
| Rhodanese | -2.0 | U35741 | 248267 | cgcgcccggc tttttagaat |
3 0 |
2 1 |
no probes | ND | |
| PAF Acetylhydrolase | -2.0 | U34277 | 93304 | ttacagaact | 0 | 0 | absent both | ND | |
| ERV1 | -2.1 | AA034842 | 27184 | gttggatagg gagggagctg |
3 0 |
3 0 |
absent both | ND | |
| Stearoyl-CoA desaturase | -2.1 | M21285 | 119597 | aacttgatac ctgatcctca |
0 0 |
0 0 |
absent both | ND | |
| PmuAUF1-3 | -2.1 | U11274 | 79625 | atgtagtagt gtgattcgca gtacccggat |
4 0 0 |
2 0 0 |
absent both | Con S | |
| HSP70 | -2.1 | U73744 | 1804142 | ccaggaggaa | 42 | 28 | NC | -1.2 | Con S |
| MDR | -2.1 | J03398 | 73812 | gcacgcatca | 0 | 0 | absent both | ND | |
| 26S proteasome component TBP-1 | -2.1 | AA145829 | 250758 | gaaggcatcc | 7 | 4 | no probes | Con S | |
| GAS3 | -2.1 | M32240 | 103724 | atcctgtcga tcgccccttc agtattgttt |
0 0 0 |
0 0 0 |
NC | +2.2 | Dis A |
| Unp ubiquitin specific protease | -2.1 | L00681 | 77500 | gacaccaact | 0 | 2 | NC | -1.1 | Dis SA |
| DNA polymerase alpha primase | -2.2 | AA1396807 | 81942 | aatcaaacac | 0 | 0 | no probes | ND | |
| ubiquitin thiolesterase | -2.2 | AA061604 | 5452 | tctgtgctgt | 0 | 0 | absent both | ND | |
| p130PITSL Cyclin-kinase | -2.2 | L37092 | 183418 | ttttgttttt | 3 | 1 | NC | +1.1 | ND S≠A |
| Calmodulin | -2.2 | AA103356 | 141011 | atccgtgccc | 5 | 2 | a)NC b)NC c)NC |
a)1 b)+1.3 c)+1.2 |
ND S≠A |
| DNA binding protein | -2.2 | L24508 | ? | ? | ? | ? | ? | ? | |
| Pro-alpha (III) collagen | -2.3 | X52046 | 119571 | ccacagggat ccacagggga |
0 2 |
0 1 |
MD | -3.5 | Con A |
| PTHR | -2.3 | L34611 | 1019 | tgaccaggcg | 0 | 0 | no probes | ND | |
| gp130 | -2.3 | M83336 | 82065 | cctcagtgaa gtacgaatgg |
0 0 |
0 0 |
absent both | ND | |
| mSin3 | -2.3 | U22394 | 22583 | gtcactgctc | 0 | 0 | no probes | ND | |
| PKCSH | -2.5 | W54288 | 1432 | aagccagccc | 1 | 4 | NC | -1.1 | Dis SA |
| Phosphoprotein phosphatase | -2.5 | M81475 | 75206 | acttagagtc | 0 | 2 | a)NC b)NC |
a)+1.1 b)+1.2 |
Dis SA |
| TR2L | -2.5 | U70210 | 15740 | ccacctgaat aagggacatt |
0 1 |
0 0 |
a)NC b)NC, absent old |
a)+1.2 b)-2.5 |
ND |
| 3f8 | -2.5 | X76652 | 49597 | ctcccaatca | 0 | 0 | no probes | ND | |
| 20S proteasome subunit | -2.6 | U22031 | 180062 | aaggaagatg | 0 | 0 | absent both | ND | |
| Phox2 homeodomain protein | -2.7 | X75014 | 247684 | gaggcgtccg | 0 | 0 | no probes | ND | |
| BMP8b | -2.8 | U39545 | 99948 | gtggtcaagg | 0 | 0 | absent both | ND | |
| Desintegrin-related protein | -2.8 | U06146 | 174030 | ttagcactgc aatttaaaat |
0 0 |
0 0 |
absent both | ND | |
| Alpha enolase | -2.9 | W550371 | 254105 | agaaaaaaaa tgagcctcgt |
7 0 |
7 1 |
no probes | ND | |
| EF-1-gamma | -2.9 | AA107752 | 2186 | tgggcaaagc | 163 | 146 | no probes | Dis S | |
| Necdin | -2.9 | D76440 | 50130 | accttgctgg | 0 | 0 | absent both | ND | |
| Ox40 | -3.0 | X85214 | 129780 | catacctcct | 2 | 0 | absent both | Con S | |
| Clathrin light chain B | -3.1 | AA138226 | 73919 | ggctgggcct gctgccggca |
5 0 |
5 2 |
a)NC b)absent both |
a)-1.1 | Dis A |
| Pro-alpha-2(I) collagen | -3.1 | X58251 | 179573 | tttggttttc | 2 | 0 | a)MD, absent old b)NC, absent old c)NC d)absent both |
a)-1.3 b)1 c)-1.4 |
Con S |
| Alpha 1 type 1 collagen | -3.2 | U08020 | 172928 | accaaaaacc tggaaatgac |
0 1 |
0 0 |
absent both | ND | |
| Ovalbumin transcription factor I | -3.2 | X741349 | 144630 | ggaaaaagag tccatccagt agaattttaa |
0 0 0 |
0 0 0 |
no probes | ND | |
| Calpactin I light chain | -3.4 | M16465 | 119301 | agcagatcag | 3 | 3 | NC | 1 | Dis A |
| Wnt-4 | -3.5 | M89797 | 146409 | tctcaattct cactcgtgtg ccttgctttt |
1 0 0 |
2 2 2 |
absent both | Dis S | |
| Protein phosphatase PP2a | -3.6 | AA162443 | 28219 | aacagagaga ttgaaaacaa actttgtttt10 |
0 0 4 |
0 0 4 |
NC | +1.2 | Dis A |
| Mitochondrial LON protease | -4.1 | AA061310 | 278614 | aatctctaat actggcgaag tgaatgatac |
0 2 0 |
0 4 1 |
no probes | Dis S | |
| Albumin gene D-box binding protein | -4.3 | U29762 | 155402 | aagaaggcaa cgcgtcctcc |
0 0 |
1 0 |
a)absent both b)NC |
b)+9.6 | Dis A |
| Protein tyrosine phosphatase, PTPBR7 | -4.4 | D31898 | 198288 | tatgtcctta | 0 | 0 | no probes | ND | |
| Myosin heavy chain, perinatal | -4.9 | AA106126 | 113973 | agctggagga11 | 5 | 1 | unusable probe set11 | Con S | |
| Squalene synthase | -6.4 | D29016 | 48876 | agatcctact cttttggctg |
3 1 |
5 0 |
NC | 1 | Dis A |
Alpha-Amylase-1 is not included because it was listed as being overexpressed in older muscle in the online tables but was listed as being underexpressed in older muscle in the paper of Lee et al. (15).
1Indicates sequence withdrawn from GenBank, searched by gene name.
2This UniGene cluster is the best match for both AA114576, which was expressed at an increased level in old murine muscle, and U73744, which was expressed at a reduced level in old murine muscle.
4These are the alpha-2 and alpha-1 human globin genes, which have the same coding sequence but different 3'UTR sequences.
5The published mRNA sequences do not have a SAGE tag (no CATG); this tag was found in several ESTs in this UniGene cluster.
6Best match is a mitochondrial gene, ATP synthase 6/8. UniGene does not have clusters for mitochondrial genes. Although there are several non-mitochondrial human ESTs matching this mouse ORF, there was concern about contamination by mouse cDNA.
7Used gene name rather than ORF to identify homologous human gene because of poor homology between this ORF and human mRNA/EST sequences in GenBank.
8Entrez found no entry for L2450. Too many genes matched "DNA binding protein" to find human homolog by gene name.
9This ORF is also given in a separate row in the online table of Lee et al., with the gene name COUP-TFI. Both genes are listed as being about 3-fold more abundant in young muscle.
10Also tag for ESTs in UniGene Hs.143446.
11The tag for human perinatal myosin is given here even though the BLAST scores for other isoforms are higher. As reported elsewhere (29), older muscle had less type 2a myosin heavy chain than young, but the amount of type 1 was not affected by aging. The Affymetrix data for this transcript do not appear to be meaningful because of the very high levels of other myosin isoforms, which cause very high nonspecific hybridization and a negative score for abundance even in the sample with higher abundance.
12Decisions were concordance (Con) between mice and men in the effect of age on expression of the gene, discordance (Dis) between mice and men in the effect of age on expression of the gene, or no decision (ND) because of insufficient data. S indicates decision based on SAGE, A indicates decision based on array, SA indicates decision based on both methods, S≠A indicates conflict between SAGE and array results.
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