Supplemental Tables

Two supplemental tables in PDF format.

Files in this Data Supplement:

• Table S1 - Microarray analysis of the differential gene expression patterns between pachytene spermatocytes (Spcy) and round spermatids (Sptd). The 160 differentially expressed genes are reported with a fold change of expression from the triplicate experiments ± SD. Red color highlights the genes that expressed preferentially in Spcy, and green color denotes the genes preferentially expressed in Sptd. Fold change of gene expression is also color-coded. Information of cDNA sources is based on the mouse Unigene clusters Build#134 (24 Feb 2004). Experiment 3 represents the dye-swapping experiment and expression fold ratio was adjusted to present in the same way as the first two experiments.
• Table S2 - Comparison of the 15,000 cDNA microarray result with other testicular/germ cell transcriptome data. The 160 genes identified in the cDNA microarray experiment were compared with the following testicular/germ cell transcriptome analyses to obtain the concordance level among different platforms: 1. SAGE analysis of the same stages of isolated male germ cells (Wu SM et al., 2004); 2. Affymetrix GeneChip analysis of whole testes derived from different ages of mice (Schultz N et al., 2003); 3. In situ hybridization result on whole testes derived from different ages of mice (Almstrup K et al., 2004). The information available from the in situ hybridization study was insufficient for a thorough comparison with the other datasets. The red and green colors highlighted genes showing concordant change in expression with respect to the cDNA microarray result. Abbreviations used in Affymetrix GeneChip data: Abs (no expression detected); NR (genes not represented on the GeneChips); # (number of probe sets represented on the GeneChips for that particular gene).