Supplemental Figures

Resequencing for polymorphism discovery at Agtr1a from SPRET, BPL (blood pressure low), and BPH (blood pressure high) mouse strains. Bold bases/amino acids in dark gray boxes are shared across ¡Ý2 strains. Dashes are gaps (insertions/deletions between strains). Multiple sequence alignments were performed in ClustalW (MacVector, Accelerys, San Diego, CA).

Files in this Data Supplement:

• Figure A - promoter. The orientation is 5' to 3' upstream of the cap (mRNA initiation, exon 1) site. The base strain for these comparisons is SPRET, since this was the longest promoter sequence. Promoter lengths shown are SPRET, 1184 bp; BPL/1, 1112 bp; and BPH/2, 1107 bp. Note the simple sequence repeat in all 3 promoters (SPRET, -575/-912 bp; BPL/1, -577/-840 bp; BPH/2, -576/-835 bp). In the numbering scheme of the multiple sequence alignment diagram shown, the simple sequence repeat is found from +278/+608 bp. The TATA box (TATAAATAT) is at bases -36/-44 bp [+1141/+1149 bp in the diagram] upstream from the cap site. 22 single nucleotide polymorphisms (6 intra-species; 5 in BPL/1, one in BPH/2) differentiate the strains.
• Figure B - noncoding exon 1. Numbering begins with the cap (mRNA initiation) site. The extent of exon one (135 bp) is denoted by the top row ("Mouse Agtr1a Exon 1"); thereafter begins intron A. The base sequence for these comparisons is the mouse genomic chromosome 13 contig (Celera). 3 non-coding single nucleotide polymorphisms in exon 1 differentiate the strains, while there were 2 SNPs differentiating the strains in the 138 bp of intron A sequenced.
• Figure C - noncoding exon 2. Numbering begins with the first base sequenced in intron A, flanking the 5' end of exon 2. The extent of exon 2 (84 bp) is denoted by the top row ("Mouse Agtr1a Exon 2"); thereafter begins intron B. The base sequence for these comparisons is the mouse genomic chromosome 13 contig (Celera). 3 non-coding single nucleotide polymorphisms in exon 2 (all in SPRET) differentiate the strains.
• Figure D - exon 3 (the coding exon). Numbering begins with the first base in that exon. The extent of exon 3 (1960 bp in BPL/1 and BPH/2, 1966 bp in SPRET) is denoted by the second row ("Mouse Agtr1a Exon 3"). The top row ("Mouse Agtr1a ORF") denotes the 1077 bp open reading frame (ORF). The base sequence for these comparisons is the mouse genomic chromosome 13 contig (Celera). 26 single nucleotide polymorphisms in exon 3 (all in SPRET) differentiate the strains; 21/26 were in the 883 bp non-coding portions of exon 3, while 5/26 were located within the 1077 bp ORF (all 5/26 synonymous cSNPs). SPRET also exhibited one insertion (GCCCCCCGCC) and one deletion (GCGT) in the 3' non-coding region of exon 3.