Physiol. Genomics Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 8: 23-32, 2002. First published November 15, 2001; doi:10.1152/physiolgenomics.00058.2001
1094-8341/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
8/1/23    most recent
00058.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (34)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by JIANG, C.
Right arrow Articles by GREGORY, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by JIANG, C.
Right arrow Articles by GREGORY, R. J.
Received 16 July 2001; accepted in final form 13 November 2001.
Physiological Genomics 8:23-32 (2002)
1094-8341/02 $5.00 © 2002 American Physiological Society

Gene expression profiles in human cardiac cells subjected to hypoxia or expressing a hybrid form of HIF-1{alpha}

CANWEN JIANG , HSIENWIE LU , KAREN A. VINCENT , SRINIVAS SHANKARA , ADAM J. BELANGER , SENG H. CHENG , GEOFFREY Y. AKITA , RALPH A. KELLY , MARK A. GOLDBERG and RICHARD J. GREGORY

Genzyme Corporation, Framingham, Massachusetts 01701

The cellular response to hypoxia depends on rapid posttranslational modifications of proteins as well as regulation of gene expression. We performed serial analysis of gene expression (SAGE) on human cardiac cells under normoxia, subjected to hypoxia, or infected with Ad2/HIF-1{alpha}/VP16 (an adenoviral vector expressing a stable hybrid form of hypoxia-inducible factor 1{alpha}) or Ad2/CMVEV (an empty vector). Of the 97,646 SAGE tags that were sequenced, 27% matched GenBank entries, while an additional 32% matched expressed sequence tags (ESTs) in UniGene. We analyzed 161 characterized genes or ESTs with a putative identification. Expression of 35, 11, and 46 genes was increased by hypoxia, infection with Ad2/EVCMV, or infection with Ad2/HIF-1{alpha}/VP16, respectively, compared with normoxia; conversely, 20, 11, 38 genes, respectively, were expressed at lower levels. Genes regulated by hypoxia were associated with transcription, biosynthesis, extracellular matrix formation, glycolysis, energy production, cell survival, and cell stress. Changes following infection with Ad2/HIF-1{alpha}/VP16 mimicked the hypoxic response to a certain extent. Infection with Ad2/CMVEV affected expression of genes that were associated with extracellular matrix formation and membrane trafficking. Differential expression of select genes was confirmed using TaqMan in additional human cardiac cells and rat neonatal ventricular myocytes. These data provide insight into gene expression underlying the diverse and complex cellular response to hypoxia, expression of HIF-1{alpha}/VP16, or adenoviral infection.

serial analysis of gene expression; adenoviral vector




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
R. Due-Andersen, U. Pedersen-Bjergaard, T. Hoi-Hansen, N. V. Olsen, C. Kistorp, J. Faber, F. Boomsma, and B. Thorsteinsson
NT-pro-BNP during hypoglycemia and hypoxemia in normal subjects: impact of renin-angiotensin system activity
J Appl Physiol, April 1, 2008; 104(4): 1080 - 1085.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. C. Wendler, S. Amatya, C. McClaskey, S. Ghatpande, B. B. Fredholm, and S. A. Rivkees
A1 adenosine receptors play an essential role in protecting the embryo against hypoxia
PNAS, June 5, 2007; 104(23): 9697 - 9702.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
S. Rajagopalan, J. Olin, S. Deitcher, A. Pieczek, J. Laird, P. M. Grossman, C. K. Goldman, K. McEllin, R. Kelly, and N. Chronos
Use of a Constitutively Active Hypoxia-Inducible Factor-1{alpha} Transgene as a Therapeutic Strategy in No-Option Critical Limb Ischemia Patients: Phase I Dose-Escalation Experience
Circulation, March 13, 2007; 115(10): 1234 - 1243.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
Y. Luo, C. Jiang, A. J. Belanger, G. Y. Akita, S. C. Wadsworth, R. J. Gregory, and K. A. Vincent
A Constitutively Active Hypoxia-Inducible Factor-1{alpha}/VP16 Hybrid Factor Activates Expression of the Human B-Type Natriuretic Peptide Gene
Mol. Pharmacol., June 1, 2006; 69(6): 1953 - 1962.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. E. Wilhide and W. K. Jones
Potential Therapeutic Gene for the Treatment of Ischemic Disease: Ad2/Hypoxia-Inducible Factor-1{alpha} (HIF-1)/VP16 Enhances B-Type Natriuretic Peptide Gene Expression via a HIF-1-Responsive Element
Mol. Pharmacol., June 1, 2006; 69(6): 1773 - 1778.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. Demolombe, C. Marionneau, S. Le Bouter, F. Charpentier, and D. Escande
Functional genomics of cardiac ion channel genes
Cardiovasc Res, August 15, 2005; 67(3): 438 - 447.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
K. Azarnoush, A. Maurel, L. Sebbah, C. Carrion, A. Bissery, C. Mandet, J. Pouly, P. Bruneval, A. A. Hagege, and P. Menasche
Enhancement of the functional benefits of skeletal myoblast transplantation by means of coadministration of hypoxia-inducible factor 1{alpha}
J. Thorac. Cardiovasc. Surg., July 1, 2005; 130(1): 173 - 179.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. R. Raval, K. W. Lau, M. G. B. Tran, H. M. Sowter, S. J. Mandriota, J.-L. Li, C. W. Pugh, P. H. Maxwell, A. L. Harris, and P. J. Ratcliffe
Contrasting Properties of Hypoxia-Inducible Factor 1 (HIF-1) and HIF-2 in von Hippel-Lindau-Associated Renal Cell Carcinoma
Mol. Cell. Biol., July 1, 2005; 25(13): 5675 - 5686.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Date, S. Mochizuki, A. J. Belanger, M. Yamakawa, Z. Luo, K. A. Vincent, S. H. Cheng, R. J. Gregory, and C. Jiang
Expression of constitutively stable hybrid hypoxia-inducible factor-1{alpha} protects cultured rat cardiomyocytes against simulated ischemia-reperfusion injury
Am J Physiol Cell Physiol, February 1, 2005; 288(2): C314 - C320.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Frantz, K. A. Vincent, O. Feron, and R. A. Kelly
Innate Immunity and Angiogenesis
Circ. Res., January 7, 2005; 96(1): 15 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Yamakawa, L. X. Liu, A. J. Belanger, T. Date, T. Kuriyama, M. A. Goldberg, S. H. Cheng, R. J. Gregory, and C. Jiang
Expression of angiopoietins in renal epithelial and clear cell carcinoma cells: regulation by hypoxia and participation in angiogenesis
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F649 - F657.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Yamakawa, L. X. Liu, T. Date, A. J. Belanger, K. A. Vincent, G. Y. Akita, T. Kuriyama, S. H. Cheng, R. J. Gregory, and C. Jiang
Hypoxia-Inducible Factor-1 Mediates Activation of Cultured Vascular Endothelial Cells by Inducing Multiple Angiogenic Factors
Circ. Res., October 3, 2003; 93(7): 664 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. J. Prior, J. M. Hagberg, D. A. Phares, M. D. Brown, L. Fairfull, R. E. Ferrell, and S. M. Roth
Sequence variation in hypoxia-inducible factor 1{alpha} (HIF1A): association with maximal oxygen consumption
Physiol Genomics, September 29, 2003; 15(1): 20 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
W. D. Patino, O. Y. Mian, and P. M. Hwang
Serial Analysis of Gene Expression: Technical Considerations and Applications to Cardiovascular Biology
Circ. Res., October 4, 2002; 91(7): 565 - 569.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online