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Physiol. Genomics 7: 55-63, 2001;
1094-8341/01 $5.00
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Received 15 December 2000; accepted in final form 9 August 2001.
Physiological Genomics 7:55-63 (2001)
1094-8341/01 $5.00 © 2001 American Physiological Society

DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress

BENJAMIN P. C. CHEN1, YI-SHUAN LI1, YIHUA ZHAO1, KUANG-DEN CHEN1, SONG LI1, JIANMIN LAO1, SULI YUAN1, JOHN Y.-J. SHYY2 and SHU CHIEN1

1 Department of Bioengineering and the Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093-0427
2 Division of Biomedical Sciences, University of California, Riverside, Riverside, California

The recently developed DNA microarray technology provides a powerful and efficient tool to rapidly compare the differential expression of a large number of genes. Using the DNA microarray approach, we investigated gene expression profiles in cultured human aortic endothelial cells (HAECs) in response to 24 h of laminar shear stress at 12 dyn/cm2. This relatively long-term shearing of cultured HAECs led to the modulation of the expression of a number of genes. Several genes related to inflammation and EC proliferation were downregulated, suggesting that 24-h shearing may keep ECs in a relatively noninflammatory and nonproliferative state compared with static cells. Some genes were significantly upregulated by the 24-h shear stress; these includes genes involved in EC survival and angiogenesis (Tie2 and Flk-1) and vascular remodeling (matrix metalloproteinase 1). These results provide information on the profile of gene expression in shear-adapted ECs, which is the case for the native ECs in the straight part of the aorta in vivo.

DNA microarray; endothelial cells; gene expression; shear stress




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